Anticancer effect of Padina arborescens in oral squamous cell carcinoma via p21-mediated cellular senescence : 부챗말의 구강 편평세포암 항암효과와 p21 매개 세포노화 기전
- 주제(키워드) 도움말 Padina arborescens , oral squamous cell carcinoma , DNA damage , cellular senescence , anticancer agent , natural extract
- 발행기관 강원대학교 일반대학원
- 지도교수 도움말 최종호
- 발행년도 2026
- 학위수여년월 2026. 6
- 학위명 석사
- 학과 및 전공 도움말 일반대학원 치의학과
- 세부분야 해당없음
- 실제URI http://www.dcollection.net/handler/kangnung/000000012502
- UCI I804:42001-000000012502
- 본문언어 영어
목차 도움말
Ⅰ. Introduction 1
Ⅱ. MATERIALS AND METHODS 7
1. Preparation of P. arborescens enzymatic extract 7
2. Chemical composition analysis of PAEH 7
3. Monosaccharide composition analysis 8
4. Cell culture 8
5. Drug treatment 9
6. 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl tetrazolium bromide（MTT） assay 9
7. Western blot 10
8. 5-Ethynyl-2’-deoxyuridine （EdU） assay 10
9. Terminal deoxynucleotidyl transferase dUDT nick-end labeling （TUNEL） assay 11
10. Senescence-associated beta-galactosidase （SA-β-gal）staining 11
11. Senescence-Associated-β-galactosidase green staining 12
12. Wound healing assay 13
13. Transwell assay 13
14. Immunofluorescence 14
15. In vivo xenograft and metastasis model 14
16. Human Alu real-time PCR primer 15
17. Immunohistochemistry 16
18. Statistical analysis 16
Ⅲ. RESULTS 18
1. Characteristics of PAE 18
2. PAE inhibits cell growth in OSCC cell line 21
3. PAE regulates the DNA damage response pathway and cell cycle in OSCC cell line 25
4. PAE induces cell death and cellular senescence in OSCC cell line 34
5. Effects of PAE in normal gingival keratinocyte cell line 46
6. PAE inhibits tumor growth and metastasis of OSCC cell line in vivo 49
Ⅳ. DISCUSSION 57
Ⅴ. CONCLUSION 62
REFERENCES 64
국문 ABSTRACT 75
