A New Peptide Discovery from the Co-culture of Aspergillus fumigatus and Streptomyces tendae
Aspergillus fumigatus와 Streptomyces tendae 공배양을 통한 신규 펩타이드 발굴
- 주제(키워드) 도움말 co-culture , Microbial interaction , Streptomyces tendae , Aspergillus fumigatus , VVYW , Linear tetrapeptide , Biotransformation
- 발행기관 강원대학교 일반대학원
- 지도교수 도움말 박현봉
- 발행년도 2026
- 학위수여년월 2026. 6
- 학위명 석사
- 학과 및 전공 도움말 일반대학원 생물학과
- 세부분야 해당없음
- 실제URI http://www.dcollection.net/handler/kangnung/000000012475
- UCI I804:42001-000000012475
- 본문언어 영어
목차 도움말
I. Introduction 1
II. Materials and Methods 3
1. Small-Scale Culture and Screening of Co-culture-Specific Metabolite Features 3
2. Large-Scale Cultivation, Isolation, and Purification 5
3. UV and NMR Spectroscopic Analysis 5
4. Acid Hydrolysis and Marfey’s Analysis 6
5. Solid-Phase Peptide Synthesis of Stereoisomeric VVYW Standards 6
6. Cross-treatment of Microbial Metabolite Extracts 7
7. Detection of VVYW Formation after Compound 2 Treatment 7
8. Disk diffusion assay 7
9. Minimum Inhibitory Concentration Assay 7
10. Tyrosinase Inhibition Assay 8
III. Results 9
1. Screening of Co-culture-specific Metabolite Features and Isolation of Compound 1 9
2. Structural Elucidation of Compound 1 14
3. Determination of the Absolute Configuration by Marfey’s Analysis and Comparison 19
4. Biological Activity Evaluation of Compound 1 22
5. Detection and Characterization of a Putative –Derived Cyclic Tetrapeptide Candidate 23
6. Comparison of Growth Inhibitory Activity of Compound 2 and VVYW against S. tendae 28
7. Confirmation of VVYW Formation after Treatment of with Compound 2 29
IV. Discussion 31
V. References 33
국문요약 35
