Apoptosis and Autophagy Inducing Effects of a New Quinone Compound on HCT116 Colorectal Cancer Cells
HCT116 대장암 세포에서 신규 퀴논 화합물에 의한 세포자멸사 및 자가포식 유도
- 주제(키워드) 도움말 1 , 4-naphthoquinone , Microbial metabolites , Colorectal Cancer , apoptosis , autophagy
- 발행기관 강원대학교 일반대학원
- 지도교수 도움말 박현봉
- 발행년도 2026
- 학위수여년월 2026. 6
- 학위명 석사
- 학과 및 전공 도움말 일반대학원 생물학과
- 세부분야 해당없음
- 실제URI http://www.dcollection.net/handler/kangnung/000000012473
- UCI I804:42001-000000012473
- 본문언어 영어
목차 도움말
I. Introduction 1
II. Material and Methods 4
1. Cell cultures 4
2. WST assay 4
3. Colony formation assay 4
4. Cell cycle analysis 5
5. Annexin Ⅴ/PI staining 5
6. Intracellular ROS measurement 5
7. RT-qPCR 5
8. Western blot analysis 6
9. RNA sequencing 7
10. siRNA transfection 8
11. Statistical Analysis 9
III. Results 10
1. Cytotoxic effect of amiquinone on HCT116 cells 10
2. Inhibition of HCT116 cell proliferation by amiquinone 11
3. Cell cycle arrest induced by amiquinone in HCT116 cells 12
4. Amiquinone induces apoptotic cell death in HCT116 cells 13
5. Activation of apoptotic markers by amiquinone 14
6. Transcriptomic analysis reveals distinct gene expression patterns after amiquinone treatment 15
7. Amiquinone upregulates genes associated with multiple stress and cell death pathways 16
8. GSEA suggests enrichment of stress response and apoptosis pathways in cells treated with amiquinone 18
9. Intracellular ROS accumulation is increased by amiquinone 19
10. Genes involved in the UPR are upregulated following amiquinone treatment 20
11. JNK and ERK signaling are activated in response to amiquinone 21
12. Amiquinone upregulates TNFRSF10B/DR5 expression at the mRNA and protein levels 22
13. TNFRSF10B knockdown reduces apoptotic signaling induced by amiquinone 23
14. Amiquinone regulates autophagy markers at the transcript and protein levels in HCT116 cells 24
IV. Discussion 26
V. Reference 29
