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가리비류(Scallops)의 인공종자생산에 미치는 수온 및 먹이생물의 영향

Effects of water temperature and food organisms on artificial seed production of scallops

초록/요약 도움말

In order to develop the optimal water temperature and feed organisms for the artificial seed production of scallops, a basic study was conducted on the effects of temperature and feed on spawning season, larval development, and the growth of larvae and spat. The results are as follows. 1. Yesso scallop (Mizuhopecten yessoensis) The Yesso scallops (Mizuhopecten yessoensis) used in this study ranged from 89.2 to 126.7 ㎜ in shell length (mean 111.3 ㎜), with a total weight ranging from 82.1 to 256.3 g (mean 161.8 g). The relationship between shell length (SL) and shell height (SH) was expressed as SH=0.9172SL+6.1330 (r2=0.9532), while the relationship between shell length (SL) and total weight (TW) was represented by an exponential equation, TW=0.0001SL2.9419 (r2=0.9081). The gonads of female Yesso scallops were reddish, whereas those of males were milky white. The spawning season was presumed to be from April to June. A total of 584 million eggs were obtained, with fertilization rates ranging from 76.5 to 86.4% (average 81.7%) and hatching rates ranging from 81.4 to 90.6 (average 85.9%). The fertilized egg of the Yesso scallop were a spherical, with a diameter of 76.4 ㎛. At a constant water temperature of 16℃, it took 5 hours and 40 minutes from fertilization to become 4-cell stage, 3 days to become D-shape larvae, 15 days to become umbo stage and 25 days to become fully grown veliger ready for settlement. The biological minimum water temperature at which ovarian development did not progress in the initial development of the common scallop was 3.67℃. Larval growth was optimal at water temperatures of 16∼19℃. When reared on single diets, larvae showed the highest growth when fed Isochrysis gslbana. For mixed diets, supplying a mixture of three foods (I. galbana+P. lutheri+C. calcitrans) for 20 days resulted in the highest larval size of 252.4 ㎛, whereas the mixture of two foods (C. calcitrans+P. lutheri) produced the lowest size of 225.6㎛. The growth of scallop spat reached 343㎛ on 5 days after attachment, 496 ㎛ on 10 days, and exhibited rapid growth to 1,596 ㎛ by 30 days. 2. Jicon scallop (Chlamys farreri) The shell length of the Jicon scallop ranged from 54.3 to 76.8 ㎜ (mean 65.4 ㎜), and the total weight ranged from 29.1 to 52.9 g (mean 41.6 g). The relationship between shell length (SL) and shell height (SH) was expressed as SH=0.9842SL+3.9440 (r2=0.9351), while the relationship between shell length (SL) and total weight (TW) was represented by an exponential equation, TW=0.0105SL1.9793 (r2=0.9153). The gonads of female Jicon scallops were pink in color, whereas those of males were milky or yellowish-white. The spawning season was presumed to be from May to July. Amount of spawning of 296 milion eggs were obtained, with fertilization rates ranging from 81.5 to 94.3% (average 88.9%) and hatching rates ranging from 88.5 to 93.2% (average 91.4%). The fertilized egg of the Jicon scallop were a spherical, with a diameter of 69.2 ㎛. At a constant water temperature of 18℃, it took 3 hours and 10 minutes from fertilization to become 4-cell stage, 41 hours to become D-shape larvae, 12 days to become umbo stage and 20 days to become fully grown veliger ready for settlement. The biological minimum water temperature at which ovarian development did not progress in the initial development of the common scallop was 6.01℃. Larval growth was optimal at water temperatures of 18∼21℃. When reared on single diets, larvae showed the highest growth with I. galbana (213.6 ㎛). For mixed diets, supplying a mixture of three foods (I. galbana+P. lutheri+C. calcitrans) for 16 days resulted in the highest larval size of 229.3 ㎛, whereas the mixture of two foods (C. calcitrans+P. lutheri) produced the lowest size of 204.5 ㎛. The growth of scallop spat reached 298 ㎛ on 5 days after attachment, 395 ㎛ on 10 days, and exhibited rapid growth to 1,169 ㎛ by 30 days. 3. Bay scallop (Argopecten irradians) The shell length of the Bay scallop ranged from 69.4 to 86.6 ㎜ (mean 78.2 ㎜), and the total weight ranged from 30.1 to 45.8 g (mean 36.6 g). The relationship between shell length (SL) and shell height (SH) was expressed as SH=0.9351SL+0.0103 (r2=0.8304), whill the relationship between shell length (SL) and total weight (TW) was represented by an exponential equation, TW=0.0316SL1.6176 (r2=0.9035). The Bay scallop was a hermaphroditic species, with the female gonads were orange in color, while those of the male were milky white. The spawning season was presumed to be from April to June. Amount of spawning of 284 million eggs were obtained, with fertilization rates ranging from 84.6 to 93.2% (average 88.9%) and hatching rates ranging from 89.8 to 95.5% (average 93.1%). The fertilized egg of the Bay scallop were spherical, with a diameter of 61.3 ㎛. At a constant water temperature of 22℃, it took 2 hours from fertilization to become 4-cell stage, 32 hours to become D-shape larvae, 5 days to become umbo stage and 11 days to fully grown veliger ready for settlement. The biological minimum water temperature at which ovarian development did not progress in the initial development of the common scallop was 6.91℃. Larval growth was optimal at water temperatures of 19∼22℃. When reared on single diets, larvae showed the highest growth with I. galbana (206.6 ㎛), For mixed diets, supplying a mixture of three foods (I. galbana+P. lutheri+C. calcitrans) for 12 days resulted in the highest larval size of 223.3 ㎛, whereas the mixture of two food (C. calcitrans+P. lutheri) produced the lowest size of 200.2 ㎛. The growth of scallop spat reached 250 ㎛ on 5 days after attachment, 321 ㎛ on 10 days, and exhibited rapid growth to 752 ㎛ by 30 days.

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목차 도움말

Ⅰ. 총 서론 1
Ⅱ. 큰가리비 3
1. 서 론 3
2. 재료 및 방법 4
2-1. 모패의 외부 형태 및 생태 4
2-2. 성숙도 조사 4
2-3. 산란유발 5
2-4. 난 발생 및 유생의 발달 5
2-5. 유생의 성장과 생존 5
2-6. 채묘 및 치패 사육 7
2-7. 통계처리 8
3. 결 과 9
3-1. 모패의 형태 및 생태 9
3-2. 성숙도 조사 14
3-3. 산란유발 17
3-4. 난 발생 및 유생의 발달 18
3-5. 유생 성장과 생존율 26
3-6. 부착기질에 따른 채묘 시험 42
3-7. 치패 사육 43
4. 고 찰 47
Ⅲ. 비단가리비 55
1. 서 론 55
2. 재료 및 방법 57
2-1. 모패의 외부 형태 및 생태 57
2-2. 성숙도 조사 57
2-3. 산란유발 57
2-4. 난 발생과 유생의 발달 57
2-5. 유생의 성장과 생존 58
2-6. 채묘 및 치패 사육 59
2-7. 통계처리 59
3. 결 과 60
3-1. 모패의 형태 및 생태 60
3-2. 성숙도 조사 66
3-3. 산란유발 69
3-4. 난 발생 과정 및 유생의 발달 70
3-5. 유생 성장과 생존율 78
3-6. 부착기질에 따른 채묘 시험 94
3-7. 치패 사육 95
4. 고 찰 99
Ⅳ. 해만가리비 106
1. 서 론 106
2. 재료 및 방법 108
2-1. 모패의 외부 형태 및 생태 108
2-2. 성숙도 조사 108
2-3. 산란유발 108
2-4. 난 발생 및 유생의 발달 108
2-5. 유생의 성장과 생존 109
2-6. 채묘 및 치패 사육 110
2-7. 통계처리 110
3. 결 과 111
3-1. 모패의 형태 및 생태 111
3-2. 성숙도 조사 117
3-3. 산란유발 120
3-4. 난 발생 및 유생의 발달 121
3-5. 유생 성장과 생존 129
3-6. 부착기질에 따른 채묘 시험 145
3-7. 치패 사육 146
4. 고 찰 150
Ⅴ. 결 론 157
Ⅵ. 요 약 166
Ⅶ. 참고문헌 169

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