대서양연어의 절창병 예방을 위한 경구백신의 분자면역학적 기작 연구
Molecular immunological mechanisms of an oral vaccine for the prevention of Furunoculosis in Atlantic salmon
- 주제(키워드) 도움말 oral vaccine
- 발행기관 국립강릉원주대학교 일반대학원
- 지도교수 도움말 홍수희
- 발행년도 2025
- 학위수여년월 2026. 2
- 학위명 석사
- 학과 및 전공 도움말 일반대학원 수산생명의학과
- 실제URI http://www.dcollection.net/handler/kangnung/000000012391
- UCI I804:42001-000000012391
- 본문언어 한국어
초록/요약 도움말
ASS, a gram-negative rod shaped bacteria is a well known factor for causing Furunoculosis in Atlantic salmon(Salmo salar). Many methods are invested to overcome the shortages of relying on antibiotics as the uprsing usage, abuse of antibiotics can lead to the outbreaks of antibiotic resistant bacteria which can be a potentional threat. Injection and immersion are the most well-known next level methods against this threat, but they also show some limitations by different conditions such as fish size and immune response persistence. This is the point that the oral vaccination gets focused due to its advantages of showing easy and efficient applications. But oral vaccinatons also show shortages, especially with antigen delivery. Antigen alone cannot be safely loaded to the mucosal parts of the intestine through the stomach. Alginate sodium and Chitosan are well known to work as an adjuvant to protect the antigens stable. In this study, Alginate sodium and Chitosan was used to make an oral vaccine and tested. Immune parameter changes were then assessed to estimate its activation.
more목차 도움말
Ⅰ. 서론 1
Ⅱ. 재료 및 방법 5
1. Fish 5
2. 균 6
3. 알긴산 및 키토산, TPP를 사용한 미세구슬 제작 7
4. 사료와 미세구슬의 혼합 및 급이 8
5. 타임 코스 별 샘플링 9
6. 시간 코스별 수득 샘플 RNA 분리 및 ct값 평가 11
7. 통계학적 분석 12
Ⅲ. 결과 13
1. 균 배양 13
2. 알긴산-항원-키토산-TPP 미세구슬 형성 16
3. 타임 코스별 RNA 분리 및 cDNA 합성 17
4. 샘플링 조직 프라이머별 ct값 및 발현 확인 19
5. SPSS 통계 및 유의성 확인 28
Ⅳ. 고찰 33
Ⅴ. 참고문헌 39
