DNA 메타바코딩과 형태학적 분석 그리고 생활사 매개변수를 기반으로 한 대한민국 동해안 기수역 동물군의 새로운 윤충류 종 식별
Identification of new rotifer species in brackish water fauna in the Eastern Coast of Korea based on DNA metabarcoding, morphological analysis and life history parameters
- 주제(키워드) 도움말 DNA metabarcoding , eDNA , Brackish water , Lagoon , Species identification , Rotifer
- 발행기관 강릉원주대학교 일반대학원
- 지도교수 도움말 박흠기, 이영환
- 발행년도 2024
- 학위수여년월 2025. 2
- 학위명 석사
- 학과 및 전공 도움말 일반대학원 해양생태환경학과
- 세부분야 에코업-지속가능 환경·자원
- 실제URI http://www.dcollection.net/handler/kangnung/000000012113
- UCI I804:42001-000000012113
- 본문언어 한국어
초록/요약 도움말
The accurate identification of organisms is important when measuring the biodiversity of an area. Without accurate identification it is impossible to determine how many species exist in a given area. However, conventional monitoring techniques, such as visual surveys and counting individuals, are problematic due to challenges in identifying cryptic species or immature life stages. we conducted to assess the possibility of tracking unidentified species and new species using eDNA metabarcoding, as an alternative to conventional methods such as biodiversity assessments and invasive species monitoring. As a result of the eDNA metabarcoding analysis of Hwajinpo Lake, it was possible to confirm the species distribution and presence of invasive species based on the biodiversity of Hwajinpo, and furthermore, it might be possible to identify rotifers that might be unidentified species or new species. After collecting these specimens (H-SL [H-super large], H-US [H-ultra small]) in the brackish water of Lake Hwajinpo, Based on these rotifers, morphological species identification methods were conducted to increase the credibility of the possibility of unidentify species and new species, and both species of rotifers collected from Hwajinpo were identified as Brachionus genus, and it was confirmed that their sizes were significantly different from those of previously known species. Physiological comparisons also confirmed that the species collected from Hwajinpo had strong adaptability to low temperatures, and the high growth rates overall suggest that the species collected from Hwajinpo could be different species from the previously experimental species. eDNA metabarcoding is highly useful for tracking unrecorded species and new species, and It was confirmed that combining existing visual investigation methods with eDNA would improve accurate species identification. This study demonstrated the utility of eDNA as a non-invasive technique for biodiversity assessment which is particularly useful in areas like brackish water with scarce data on species distribution.
more초록/요약 도움말
본 연구는 eDNA 메타바코딩을 활용하여, 기존 생물다양성과 외래침입종 확인 등의 방법이 아닌, 미기록종, 신종 추적 가능성을 확인하고자 진행하였다. 화진포호의 eDNA 메타바코딩 분석 결과 화진포의 생물다양성을 기반으로 한 종 분포 그리고 침입종 유무 모두 확인할 수 있었으며, 더 나아가 미기록종, 신종의 가능성이 있는 윤충류도 확인할 수 있었다. 이에 미기록종, 신종 가능성의 신빙성을 높이고자 형태 동정을 진행하였으며, 실제 화진포에서 채집된 윤충류 2종 모두 Brachionus 속에 포함되는 윤충류로 식별되었고, 기존 알려진 종들과는 크기가 유의적으로 차이가 나는 것을 확인할 수 있었다. 생리학적 비교 또한 화진포 채집 종들이 저온에 강한 적응력을 지닌 것을 확인할 수 있었으며, 전반적으로 높은 성장률을 지닌 것은 화진포 채집 종들이 기존 실험 종들과는 서로 다른 종일 가능성을 제시한다. eDNA 메타바코딩은 미기록종, 신종을 추적하는데 충분히 유용하며, 정확한 종 식별에 도움을 위해 기존 시각적 조사 방법이 함께 진행되면 더욱 효과적인 것을 확인할 수 있었다. 이러한 방법은 수생태계 분포 데이터가 부족한 석호와 같은 기수지역에서 특히 유용하게 사용될 것으로 사료된다.
more목차 도움말
Ⅰ. Introduction 11
Ⅱ. Materials and Methods 13
1. Collection area 13
1.1) Hwajinpo lake 13
1.2) eDNA sampling and sample processing 13
1.3) Zooplankton sampling 13
2. Experimental species 15
2.1) Isolation and species identification 15
2.2) Culture and Maintenance 15
3. Morphological identification 15
3.1) Lorica and amictic egg measurements 15
3.2) Trophi measurements 16
4. Molecular identification 16
4.1) DNA extraction and PCR amplification 16
5. in vivo test 17
5.1) Temperature levels 17
5.1.1) Individual culture 17
5.1.2) Population culture 17
6. Statistical analysis 18
Ⅲ. Results 19
1. Comparison of diversity and abundance on eDNA sampling site 19
1.1) Diversity 19
1.1.1) Alpha diversity 19
1.1.2) Beta diversity 19
1.2) Abundance 19
1.2.1) Differential abundance 19
1.2.2) Relative abundance 19
2. Morphology identification 22
2.1) Lorica 22
2.2.1) Neonate lorica size 22
2.2.2) Adult(BMS) lorica size 22
2.2) Trophi 25
2.2.1) Measurements of trophi size 25
3. Molecular identification 28
4. in vivo effect of temperature levels on individual culture 30
4.1) Reproduction phase 30
4.1.1) Pre-reproductive phase 30
4.1.2) Reproductive phase 30
4.1.3) Post-reproductive phase 30
4.2) Lifespan 31
4.3) Offspring 31
5. in vivo effect of temperature levels on population culture 35
5.1) SGR 35
5.2) Highest density 35
Ⅳ. Discussion 39
Ⅴ. Summary 42
Ⅵ. Reference 43
