빛과 calcitriol이 무지개송어와 대서양연어의 포도당 대사 및 성장에 미치는 영향
Effecets of light and calcitriol on the glucose metabolism and growth in rainbow trout(Oncorhynchus mykiss) and Atlantic salmon(Salmo salar)
- 주제(키워드) 도움말 Oncorhynchus mykiss , Salmo salar , glucose metabolism , bone growth
- 발행기관 강릉원주대학교 일반대학원
- 지도교수 도움말 김이경
- 발행년도 2024
- 학위수여년월 2025. 2
- 학위명 석사
- 학과 및 전공 도움말 일반대학원 수산생명의학과
- 실제URI http://www.dcollection.net/handler/kangnung/000000012097
- UCI I804:42001-000000012097
- 본문언어 한국어
초록/요약 도움말
Salmonidae, including rainbow trout, are a species that has been widely attempted in many farming ventures in Korea. This study was conducted to evaluate the effect of photoperiod and calcitriol on the growth of salmonids. Rainbow trout were treated with three photoperiods(12L:12D, 24L:0D, and 0L:24D), and injected with calcitriol. Atlantic salmon were experimented on for 8 and 12 weeks by setting different illuminance levels (50, 500, 1000, 1500 lux) and photoperiods (6L:18D, 12L:12D, 18L:6D, 24L:0D). Additionally, a primary culture was performed with calcitriol. In rainbow trout, a 24L photoperiod and calcitriol showed a positive effect on their growth, while in Atlantic salmon, conditions of 1000 and 1500 lux with an 18L:6D and 24L:0D photoperiod showed a positive effect on bone tissue.
more목차 도움말
Chapter Ⅰ. Rainbow trout 1
Ⅰ. 서론 1
Ⅱ. 재료 및 방법 12
1) Fish 12
2) Experiment design 15
3) Sampling 19
4) Calculation of growth performance 19
5) Total RNA extraction and cDNA synthesis 20
6) Gene expression analysis 21
7) IGF-1 ELISA 23
8) Plasma Glucose analysis 23
9) Statistics analysis 23
Ⅲ. 결과 24
1) Growth performance 24
2) Effects of various photoperiod on glucose homeostasis
26
3) Effects of various photoperiod on bone formation 28
4) Effects of various photoperiod on IGF-1/IGF-1Rα 30
5) Effects of photoperiod/calcitriol treatment on glucose homeostasis 32
6) Effects of photoperiod/calcitriol treatment on bone formation 34
7) Effects of photoperiod/calcitriol treatment on IGF-1/IGF-1Rα
36
Ⅳ. 고찰 38
Chapter Ⅱ. Atlantic salmon
Ⅰ. 서론 38
Ⅱ. 재료 및 방법 39
1) Fish 39
2) Experiment design 42
3) Primary culture 46
4) Sampling 48
5) Calculation of growth performance 48
6) Total RNA extraction and cDNA synthesis 49
7) Gene expression analysis 50
8) Statistics analysis 51
Ⅲ. 결과 52
1) Intensity experiment 50
1-1) Growth performance 50
1-2) Effects of different light intensity on glucose homeostasi 52
1-3) Effects of different light intensity on bone formation 54
1-4) Effects of different light intensity on IGF-1Rα 56
2) Photoperiod experiment 57
2-1) Growth performance 57
2-2) Effects of different light photoperiod on glucose homeostiasis 60
2-3) Effects of different light photoperiod on bone formation 61
2-4) Effects of different light photoperiod on IGF-1Rα 63
3) Effects of different calcitiorl treatment on glucose homeostasis 64
Ⅳ. 고찰 66
참고문헌 68
