왕머루에서 콜히친 처리 시 4배체 획득 효율과 우량 4배체 왕머루의 초기 특성
Efficiency of Tetraploid Induction via Colchicine Treatment in Vitis amurensis and Initial Characteristics of Superior Tetraploid Vitis amurensis
- 발행기관 강릉원주대학교 일반대학원
- 지도교수 도움말 허재윤
- 발행년도 2024
- 학위수여년월 2025. 2
- 학위명 석사
- 학과 및 전공 도움말 일반대학원 원예학과
- 실제URI http://www.dcollection.net/handler/kangnung/000000011991
- UCI I804:42001-000000011991
- 본문언어 한국어
초록/요약 도움말
The increasing demand for sustainable grape production in South Korea highlights the urgent need to develop climate-resilient grape cultivars. Current grape cultivation is predominantly dependent on a limited number of varieties, such as Shine Muscat and Campbell Early, which are highly susceptible to environmental stressors, including drought, extreme temperatures, and frost damage. This dependence poses significant challenges to the grape industry’s ability to adapt to climate change. In contrast, Wangmeoru (Vitis amurensis), a native grape species recognized for its exceptional cold and disease resistance, high polyphenolic content, and medicinal properties, offers a promising genetic resource for breeding stress-tolerant grape varieties. To evaluate the potential of Wangmeoru for polyploidy-induced improvements in growth and stress tolerance, diploid Wangmeoru explants were subjected to various colchicine concentrations and exposure durations of colchicine. The results indicated that higher colchicine concentrations and longer exposure durations led to increased tetraploid induction rates, albeit with reduced survival rates. Optimal conditions (0.1–0.2% colchicine for 16 hours) yielded tetraploid Wangmeoru plants with significantly enhanced growth traits, including a higher number of stems, larger leaf areas, and thicker stems and leaves compared to their diploid counterparts. Further analyses revealed that tetraploid Wangmeoru exhibited significantly elevated antioxidant compound levels, with total phenolic content (TPC) and total flavonoid content (TFC) approximately doubling those observed in diploids. Enhanced DPPH radical scavenging activity and FRAP values were also recorded, suggesting that chromosomal doubling improves cellular antioxidant capacity. These results indicate that tetraploid Wangmeoru has potential applications in the development of antioxidant-rich functional grape varieties and in improving resilience to environmental stress. Drought tolerance was assessed through in vitro propagation of diploid and tetraploid Wangmeoru under simulated water scarcity using polyethylene glycol (PEG). Tetraploid Wangmeoru demonstrated greater stability in biomass changes under drought conditions, although the differences were not statistically significant, likely due to the limited duration of the experiment. Fatty acid profiling under drought stress conditions showed a relative increase in γ-linolenic acid content in tetraploids, a compound known to enhance membrane fluidity and signal transduction, thereby supporting cellular resilience to dehydration. However, most fatty acid levels exhibited no statistically significant differences between diploids and tetraploids. In conclusion, this study successfully induced tetraploidy in Wangmeoru through colchicine treatment, resulting in improved growth and antioxidant properties compared to diploids. Although significant enhancements in drought stress tolerance were not observed, further in vivo experiments with extended treatment durations could provide deeper insights into the potential of tetraploid Wangmeoru as a climate-resilient grape cultivar.
more목차 도움말
List of Table 2
ABSTRACT 3
Ⅰ. 서론 5
Ⅱ. 재료 및 방법 8
1. 식물 재료 및 시험관 내 배양 8
2. 이배체 왕머루에 대한 콜히친 처리의 효과 8
3. 체외 배양된 묘목의 배수성 결정 8
4. 화분 내 배수체의 초기 생장 특성 9
5. 항산화 활성 화합물 함량 분석 10
6. 건조 스트레스 처리 및 생체중 측정 10
7. 지방산 프로파일링 11
8. 통계분석 11
Ⅲ. 결과 및 고찰 13
1. 콜히친 처리에 따른 생존율 및 사배체 유도율 분석 13
2. 이배체 및 사배체 왕머루의 생장 특성 비교 18
3. 이배체 및 사배체 왕머루의 항산화 활성 물질 함량 비교 23
4. PEG 처리로 유도한 건조 스트레스 조건 26
5. 건조 스트레스 조건에서 이배체와 사배체 왕머루의 지방산 함량 비교 28
Ⅳ. 적요 31
Ⅴ. 참고 문헌 33
