Secondary metabolites of Pseudolysimachion pusanensis (Y. N. Lee) Y. N. Lee
부산꼬리풀 유래 이차대사산물 연구
- 발행기관 강릉원주대학교 일반대학원
- 지도교수 도움말 권학철, 박현봉
- 발행년도 2024
- 학위수여년월 2024. 8
- 학위명 박사
- 학과 및 전공 도움말 일반대학원 KIST강릉분원학.연협동과정
- 실제URI http://www.dcollection.net/handler/kangnung/000000011964
- UCI I804:42001-000000011964
- 본문언어 영어
초록/요약 도움말
ABSTRACT Secondary metabolites of Pseudolysimachion pusanensis (Y. N. Lee) Y. N. Lee Hoseong Hwang Department of Biology Korea Institute of Science and Technology Gangneung Institute Collaborative Course Graduate School Gangneung-Wonju National University With the increasing recognition that the chemical diversity of natural products is well-suited for providing future drugs
more초록/요약 도움말
it is anticipated that the use of new natural products and the discovery of drugs will further advance. Therefore
more목차 도움말
CONTENTS
ABSTRACT ⅰ
CONTENTS ⅲ
LIST OF FIGURES Vi
CHAPTER 1. Introduction and Scope of Study 1
1.1. Natural products in drug discovery 2
1.2. Family Scrophulariaceae 4
1.3. Genus Pseudolysimachion 5
1.3.1. Botanical and ethnobotanical aspects of Pseudolysimachion species 5
1.3.2. Previous biological investigations on Pseudolysimachion species 7
1.3.3. Previous phytochemical investigations on Pseudolysimachion species 7
1.4. Gastric cancer 10
1.5. Metabolomics 12
1.6. Computational annotation 14
1.7. Statement of Problem 16
CHAPTER 2. Computational annotation study on three Pseudolysimachion species 17
2.1. Introduction 18
2.2. Materials and methods 18
2.2.1. Plant materials 18
2.2.2. Extraction and partition 18
2.2.3. Gastric cancer cell culture and reagents 19
2.2.4. Cell viability assay 19
2.2.5. UHPLC-Q Exactive orbitrap MS 19
2.2.6. Computation annotation study 20
2.2.6.1. MS data preprocessing 20
2.2.6.2. Molecular networking analysis 21
2.3. Results and Discussions 22
2.2.1. Effects on diffuse-type gastric cancer of Pseudolysimachion species 22
2.3.2. Metabolites profiling 22
2.3.3. Molecular Networking Analysis of Pseudolysimachion Species 25
2.4. Conclusions 30
CHAPTER 3. Identification of isolated compounds from Pseudolysimachion pusanensis 31
3.1. Experimental 32
3.1.1. Plant material 32
3.1.2. Extraction and partitioning of the whole plant of Pseudolysimachion pusanensis 32
3.1.3. Compound isolation and structural characterization of Pseudolysimachion pusanensis 32
3.1.3.1. Column chromatography of the n-butanol-soluble extract 32
3.1.3.2. Isolation of the fraction 9 34
3.1.3.3. Isolation of the fraction 11 36
3.1.3.4. Isolation of the fraction 17 37
3.1.3.5. Isolation of the fraction 18 41
3.1.3.6. Isolation of the fraction 21 42
3.1.3.7. Isolation of the fraction 23 44
3.1.3.8. Isolation of the fraction 24 45
3.1.3.9. Isolation of the fraction 27 46
3.1.3.10. Isolation of the fraction 29 48
3.1.3.11. Isolation of the fraction 30 53
3.1.3.12. Isolation of the fraction 31 54
3.1.3.13. Isolation of the fraction 33 55
3.2. Results and Discussion 56
3.2.1. Structure elucidation and identification of the isolates from Pseudolysimachion pusanensis 56
3.2.1.1. Structure elucidation of compound 1 56
3.2.1.2. Structure elucidation of compound 4 64
3.2.1.3. Structure elucidation of compound 6 71
3.2.1.4. Structure elucidation of compound 3 78
3.2.1.5. Structure elucidation of compound 2 85
3.2.1.6. Structure elucidation of compound 5 92
3.2.1.7. Structure elucidation of compound 7 99
3.2.1.8. Structure elucidation of compound 8 106
3.2.1.9. Structure elucidation of compound 11 112
3.2.1.10. Structure elucidation of compound 10 119
3.2.1.11. Structure elucidation of compound 13 125
3.2.1.12. Structure elucidation of compound 12 132
3.2.1.13. Structure elucidation of compound 9 138
3.2.2. Diffuse-type gastric cancer cell inhibition activities of the isolates from Pseudolysimachion pusanensis 145
3.2.3. Enzymatic hydrolysis of the isolates from Pseudolysimachion pusanensis 149
3.3. Conclusions 153
CHAPTER 4. Conclusion 154
ABSTRACT IN KOREAN 156
CITED LITERATURE 157
