순환여과양식시스템에서 Ozone-Produced Oxydants 농도에 따른 넙치(Paralichthys olivaceus)의 성장과 생물위해성 평가
Evaluation of Ozone-Produced Oxydants concentration on growth and their biohazard of olive flounder, Paralichthys olivaceus in a Recirculating Aquaculture System
- 주제(키워드) 도움말 "넙치" , "오존" , "RAS"
- 발행기관 강릉원주대학교 일반대학원
- 지도교수 도움말 김이경
- 발행년도 2024
- 학위수여년월 2024. 2
- 학위명 석사
- 학과 및 전공 도움말 일반대학원 해양생명공학과
- 세부분야 해당없음
- 실제URI http://www.dcollection.net/handler/kangnung/000000011670
- UCI I804:42001-000000011670
- 본문언어 한국어
초록/요약 도움말
This study investigated effects of ozone-produced oxydants (OPO) on the growth, hematology, and histology and the changes of water quality parameters and microbial colonies when ozone was applied to a recirculating aquaculture system (RAS) for olive flounder Paralichthys olivaceus. As a result, survival rate was not significantly different between groups (P>0.05). Tissues of gills, hepato-pancreases, and kidneys sampled from ozonated tanks appeared to be affected by OPO. Also hematological examinations manifested disturbances in some blood indices with OPO. Levels of Potaassium. chloride, total porotein, alanine aminotransferase significantly changed in ozonated groups. Nitrite nitrogen, nitrate nitrogen, phosphate phosphorus except total ammonia nitrogen (TAN) decreased significantly with increasing OPO concentration in daily and weekly monitoring (P<0.05). Colony forming unit (CFU) counts of heterotrophic marine bacteria decreased OPO concentration dependently. Overall reduction rates of microbial colonies in the treatments were higher than 80% over the control (P<0.05). During the experiment, the OPO concentration-driven ozonation was reliably practiced an event of adverse effect on culture animals in RAS. Considering the biohazard, operating cost, and stability of ozonation, a OPO concentration of 0.014 mg Cl2/L would be sufficient to control water quality parameters and microbial colonies in a RAS. The present study implied that long-term exposure to OPO concentration of 0.014 mg Cl2/L and above for olive flounder grow-out may result in damages in tissues of gills, hepatopancreases, and kidneys and physiological stress, albeit no short-term apparent impact to growth and survival.
more목차 도움말
목 차
국문요약 ․․․․․․․․․․․․․․․․․․․․․․․․․․․․․ ․․1
Ⅰ. 서론 33
Ⅱ. 재료 및 방법 7
1. 실험시설
1.1 반감기 시험용 7
1.2 단기독성 시험용 10
1.3 장기독성 및 사육실험용 10
1.4 오존산화시스템 10
2. 실험방법
2.1 오존잔류산화물 반감기 10
2.2 오존잔류산화물 급성독성 10
2.3 오존잔류산화물의 장기독성 및 사육 11
1) 장기사육 실험 11
2) 일간변동 실험 12
3. 측정방법
3.1 성장과 생존률 13
3.2 혈액 조성 및 조직 분석 13
3.3 수질 및 미생물 분석 14
4. 통계처리 16
Ⅲ. 결과 17
1. 오존잔류산물의 반감기 17
2. 오존잔류산물의 단기독성 18
3. 오존잔류산물의 영향 20
3.1 성장지표 20
3.2 혈액조성 21
3.3 수질 및 미생물 26
3.4 오존잔류산화물의 시스템내 거동 31
3.5 수질 및 미생물의 실시간 거동 32
3.6 주요 장기조직의 변화 39
Ⅳ. 고찰 41
Ⅴ. 참고문헌 59
