순환여과양식시스템(RAS)에서 인위적인 광주기의 조절은 무지개송어의 성장 및 해수적응력을 향상시킬 수 있다.
- 주제(키워드) 도움말 Oncorhynchus mykiss , photoperiod , thyroid hormone , skeletal growth
- 발행기관 강릉원주대학교 일반대학원
- 지도교수 도움말 김이경
- 발행년도 2024
- 학위수여년월 2024. 2
- 학위명 석사
- 학과 및 전공 도움말 일반대학원 수산생명의학과
- 세부분야 해당없음
- 실제URI http://www.dcollection.net/handler/kangnung/000000011639
- UCI I804:42001-000000011639
- 본문언어 한국어
초록/요약 도움말
Among the environmental factors, photoperiod plays an important role in growth of fish. This study was conducted to assess the effects of photoperiod on growth and seawater adaptability of rainbow trout (Oncorhynchus mykiss), a commonly cultured freshwater fish species. The experiment was conducted with three different photoperiod treatments, such as 24 h of light (24L : 00D), 14 h of light and 10 h of dark (14L : 10D), and 24 h of dark (00L : 24D). In addition, the seawater adaptability was tested. Fish was exposed in the three photoperiod conditions and sampled fishes sacrificed to measure growth parameters (weight gain rate, WGR; specific growth rate, SGR; condition factor), survival in seawater, thyroid hormone concentration in plasma and gene expression. Growth parameters, survival in seawater, growth-related gene expression and thyroid hormone concentration was highest in 24L : 00D and lowest in 14L : 10D condition. This study indicates that continous photoperiod positive affect growth and seawater adaptability of rainbow trout.
more목차 도움말
List of Figures 1
List of Tables 4
영문요약 5
Ⅰ. 서론 6
1. The need for research of rainbow trout 6
2. Effect of photoperiod on growth and seawater adaptability 7
3. The physiology of smoltification in rainbow trout 8
4. The role of thyroid hormone in fish 9
5. Stimulation of skeletal growth by thyroid hormone in fish 12
Ⅱ. 재료 및 방법
1. Fish 19
2. Experiment design 23
1) Experiment Ⅰ 23
2) Experiment Ⅱ 25
3. Sampling 27
1) Experiment Ⅰ 27
2) Experiment Ⅱ 27
4. Calculation of growth performance 28
5. Total RNA extraction and cDNA synthesis 29
6. Gene expression analysis 30
7. Determined of thyroid hormone concentration in plasma 32
8. Sodium / iodide symporter (NIS) protein expression analysis 33
9. Statistics analysis 34
Ⅲ. 결과 35
1. Experiment Ⅰ 35
1) Growth performance 35
2) Thyroid hormone (T3, T4) concentration in plasma 37
3) Gene expression 39
2. Experiment Ⅱ 47
1) Growth performance 47
2) Thyroid hormone (T3, T4) concentration in plasma 49
3) Gene expression 51
3. Sodium / iodide symporter (NIS) protein expression in intestine of rain-bow trout 59
Ⅳ. 고찰 61
1. Effect of extended photoperiod on growth rate of rainbow trout 61
2. Effects of photoperiod in thyroid function in rainbow trout 63
3. Photoperiodic regulation of GH / IGF - 1 axis in rainbow trout 64
4. Thyroid hormone actions on bone of rainbow trout 66
5. Expression of NIS 68
Ⅴ. 참고문헌 69
Ⅵ. 감사의 글 86
