넙치(Paralichthys olivaceus)에 대한 toltrazuril 합성 유도체인 PK08의 독성 및 권장투여량 조사
Investigation of PK08 (synthetic toltrazuril derivative) toxicity and recommended dosage for olive flounder (paralichthys olivaceus)
- 주제(키워드) 도움말 Toltrazuril , Toxicity test , Olive flounder , Range-finding test , Immunotoxicity test , Hematological property
- 발행기관 강릉원주대학교 일반대학원
- 지도교수 도움말 김이경
- 발행년도 2024
- 학위수여년월 2024. 2
- 학위명 석사
- 학과 및 전공 도움말 일반대학원 수산생명의학과
- 세부분야 해당없음
- 실제URI http://www.dcollection.net/handler/kangnung/000000011634
- UCI I804:42001-000000011634
- 본문언어 한국어
초록/요약 도움말
Olive flounder, which occupies for more than 50% of Korea's farmed fish production, has recently been damaged by K. septempunctata infection, a myxospore of the genus cudoa. Due to this food safety problem, there are concerns about a decrease in exports and domestic consumption of olive flounder in Korea. Toltrazuril was developed in the 1980s for the purpose of treating and preventing parasitic diseases in livestock. So far, it has been approved as veterinary drug and is widely used in the livestock (Mehlhorn et al., 1988). In the acute toxicity test using toltrazuril on olive flounder, the lethal dose 50% (LD50) of olive flounder was about 45 times stronger than that of rat. Synthetic toltrazuril derivative, PK08 has been developed that can reduce toxicity through structural changes of toltrazuril. Based on the results of range-finding tests, it was found that PK08's no observed adverse effect level (NOAEL) for olive flounder was 120 mg/kg, the lowest observed Adverse Effect Level (LOAEL) was 150 mg/kg, and the definite toxic dose was 200 mg/kg. As a result of the immunotoxicity test, the 200 mg/kg experimental group was close to LD50, so predictable toxicity findings were found, and these results can be used as a reference to determine the effective concentration that can be used for the treatment of olive flounder infected with K. septempunctata.
more목차 도움말
1. 서론 1
2. 재료 및 방법 6
2.1 시험물질 6
2.2 Experimental fish 7
2.3 Experimental design 8
2.3.1 Experiment I. Range-finding test 8
2.3.2 Experiment II. Immunotoxicity test 10
2.3.2.1 Hematological property 11
2.3.2.2 Biochemical parameters 11
2.3.2.3 Measurement of cellularity and cell viability rate in spleen 12
2.4 The expression analysis of drug response gene 13
2.4.1 RNA extraction and cDNA synthesis 14
2.4.2 Gene expression by qRT-PCR 14
2.5 Statistical analysis 15
3. 결과 16
3.1 Experiment I. Range-finding test 16
3.2 Experiment II. Immunotoxicity test 18
3.2.1 Hematological property 21
3.2.2 Biochemical parameters 23
3.2.3 Measurement of cellularity and cell viability rate in spleen 26
3.2.4 Drug response gene expression in the P. olivaceus liver, head kidney and body kidney 28
4. 고찰 32
5. 결론 40
참고문헌 41
