숙주나물 내의 이차대사산물 생합성 경로의 주요 유전인자 동정
Identification of key genetic factors in the biosynthetic pathways of secondary metabolites in mungbeans (Vigna radiata L.) sprouts
- 주제(키워드) 도움말 mungbean sprout , secondary metabolites , UPLC , qRT-PCR , correlation analysis
- 발행기관 강릉원주대학교 일반대학원
- 지도교수 도움말 하정민
- 발행년도 2024
- 학위수여년월 2024. 2
- 학위명 석사
- 학과 및 전공 도움말 일반대학원 원예학과
- 세부분야 해당없음
- 실제URI http://www.dcollection.net/handler/kangnung/000000011611
- UCI I804:42001-000000011611
- 본문언어 한국어
초록/요약 도움말
Mungbeans (Vigna radiata L.) are one of the major legume crops in Asia, that contain higher amounts of functional substances, such as catechin, chlorogenic acid and vitexin than other legumes. Germination increases the nutritional and functional values of plant seeds. In this study, UPLC qualitative analysis and qRT-PCR were conducted to identify the correlations between the amount of secondary metabolites and the expression level of the transcripts of key enzymes in the mungbean sprouts. Total 20 secondary metabolites were identified in the mungbean sprouts extracts. Chlorogenic acid and catechin were major compounds with significant variations among 50 genotypes. In isoflavones, the major secondary metabolites of legume crops, high correlations were identified. In the result of expression analysis of transcripts, high correlations were identified between the amount of secondary metabolites and the expression level of key genes in mungbean sprouts. These results indicates that the amount of secondary metabolites are regulated in the level of transcripts of key genetic factors in the biosynthetic pathways. This study may can provide the useful resources on mungbean sprouts breeding for increased the functional substances.
more목차 도움말
List of tables ······································································································· Ⅰ
List of figures ····································································································· Ⅰ
초록 ··························································································································· Ⅱ
1. 서론 ····················································································································· 1
2. 재료 및 방법 ····································································································· 3
2.1. 식물 재료 ··································································································· 3
2.2. 이차대사산물 정량분석 ·········································································· 6
2.3. RNA 추출 및 qRT-PCR ······································································ 9
2.4. 통계분석 ····································································································· 11
3. 결과 ····················································································································· 12
3.1. 숙주나물 추출물의 이차대사산물 함량 ············································· 12
3.2. 이차대사산물 함량을 통한 HCA 및 PCA 분석 ····························· 21
3.3. 이차대사산물 간의 상관관계 분석 ····················································· 24
3.4. 이차대사산물과 유전자 발현량 간의 상관관계 분석 ···················· 27
4. 고찰 ····················································································································· 32
5. 결론 ····················································································································· 35
6. 참고문헌 ············································································································· 36
