국내에서 분리된 IHNV의 계통발생학적 및 단백질 구조 분석과 real-time PCR을 통한 정량적 검출
Phylogenetic and protein structure analysis of IHNV strains isolated in Korea and quantitative detection by real-time PCR
- 주제(키워드) 도움말 IHNV , Phylogenetic tree , Protein structure , Real-time PCR
- 발행기관 강릉원주대학교 일반대학원
- 지도교수 도움말 홍수희
- 발행년도 2023
- 학위수여년월 2023. 8
- 학위명 석사
- 학과 및 전공 도움말 일반대학원 수산생명의학과
- 세부분야 해당없음
- 실제URI http://www.dcollection.net/handler/kangnung/000000011515
- UCI I804:42001-000000011515
- 본문언어 영어
목차 도움말
Contents i
List of Tabels iii
List of Figures iv
Abstract 1
1. Introduction 3
2. Materials and Methods 8
2. 1. Cells and Viruses 7
2. 2. Plaque assay 7
2. 3. Challenge test 8
2. 4. Primers design 8
2. 5. Viral RNA extraction and cDNA synthesis 11
2. 6. Phylogenetic tree analysis 12
2. 7. 3D protein structure prediction 13
2. 8. Preparation of a reference 13
2. 9. Real-time PCR 14
2. 10. Linear regression analysis 14
2. 11. Real-time PCR sensitivity 15
2. 12 Quantitative detection of IHNV in vitro 16
2. 13. Quantitative detection of IHNV in vivo 16
3. Result 18
3. 1. Phylogenetic tree analysis of IHNV strains isolated in Korea 18
3. 2. Pathogenicity of IHNV strains 21
3. 3. 3D protein structure prediction 24
3. 4. Optimization of real-time PCR for a quantitative detection of IHNV 35
3. 5. Optimization of reverse transcription using virus specific primer 40
3. 6. Comparison of IHNV real-time PCR and plaque assay 40
3. 7. Real-time PCR sensitivity 44
3. 8. Quantitative detection of IHNV in vitro 46
3. 9. Quantitative detection of IHNV in vivo 46
4. Discussion 51
5. 국문요약 57
6. References 59
