미세조류유를 이용한 고농도 DHA 함유 re-esterified triacylglycerol의 효소적 합성 및 반응 특성 연구
Enzymatic Synthesis and Reaction Characteristics of DHA-Enriched Re-esterified Triacylglycerol from Microalgae Oil
- 주제(키워드) 도움말 DHA , fatty acid ethyl ester , urea fractionation , glycerolysis , re-esterified triacylglycerol
- 발행기관 강릉원주대학교 일반대학원
- 지도교수 도움말 신정아
- 발행년도 2022
- 학위수여년월 2023. 2
- 학위명 석사
- 학과 및 전공 도움말 일반대학원 식품가공유통학과
- 세부분야 해당없음
- 파일정보 20215032(안태용).PDF
- 실제URI http://www.dcollection.net/handler/kangnung/000000011316
- UCI I804:42001-000000011316
- 본문언어 한국어
목차 도움말
Abstract ····················································································································· Ⅰ
List of Tables ············································································································· Ⅶ
List of Figures ··········································································································· Ⅸ
Ⅰ. 서론 ······················································································································· 1
CHAPTER Ⅰ
미세조류유 (Triacylglycerol type, TAG)로부터 전환된 Microalgae Fatty Acid Ethyl Ester (FAEE) 및 Free Fatty Acid (FFA) type 기질들의 Fractionation techniques을 이용한 고농도 DHA 분획
Ⅱ. 재료 및 방법 ········································································································ 6
1. 실험 재료 ········································································································· 6
2. 실험 방법 ········································································································· 7
1) 미세조류유 (TAG type) 기질의 지방산 조성 및 화학적 특성 ·············· 7
(1) GC를 이용한 기질의 지방산 조성 분석 ····················································· 7
(2) AV, SV, Calculated IV 측정 ········································································· 8
2) 미세조류유 (TAG type)로부터 FAEE type 기질 제조 ······························· 10
(1) Chemical catalyst (KOH)를 이용한 FAEE type 기질 제조 ············· 10
(2) Enzyme catalyst (SP435)를 이용한 FAEE type 기질 제조 ·············· 11
(3) HPLC-UVD를 활용한 미세조류유 (TAG type)의 FAEE 전환률 분석···11
3) 미세조류유 (TAG type)로부터 FFA type 기질 제조 ·································· 12
4) FAEE 및 FFA type의 전환률 확인을 위한 TLC 분석 ······························· 12
5) 고농도 DHA 농축을 위한 solvent fractionation ···································· 14
6) 고농도 DHA 농축을 위한 urea fractionation ··········································· 14
7) 통계 분석 ···················································································································· 15
Ⅲ. 결과 및 고찰 ····································································································· 16
1. 미세조류유 (TAG type) 기질의 화학적 특성 (지방산 조성, AV, SV, Calculated IV)···························································································· 16
2. 미세조류유 (TAG type)로부터 제조된 FAEE type 기질의 전환률 및 지방산 조성 ···································································································· 18
1) Chemical catalyst (KOH)를 이용한 FAEE type 기질의 전환률 및 지방산 조성 ··················································································································· 18
2) Enzyme catalyst (SP435)를 이용한 FAEE type 기질의 전환률 및 지방산 조성 ··················································································································· 19
3. 미세조류유 (TAG type)로부터 제조된 FFA type 기질의 전환률 및 지방 산 조성 ···································································································· 20
4. FAEE type 기질의 solvent fractionation을 활용한 고농도 DHA 분획· 27
1) Acetone을 이용한 FAEE type기질의 solvent fractionation ················ 27
2) Hexane을 이용한 FAEE type기질의 solvent fractionation ·················· 30
5. FFA type 기질의 solvent fractionation을 이용한 고농도 DHA 분획·····32
6. FAEE type 기질의 urea fractionation에 의한 고농도 DHA 분획 ········· 33
7. FFA type 기질의 urea fractionation에 의한 고농도 DHA 분획 ·········· 34
CHAPTER Ⅱ
고농도 DHA-FAEE를 이용한 re-esterified triacylglycerol (rTG)의 합성
Ⅳ. 재료 및 방법 ····································································································· 46
1. 실험 재료 ····································································································· 46
2. 실험 방법 ····································································································· 46
1) rTG 합성을 위한 고농도 DHA-FAEE 기질의 scale-up 제조 ·················· 46
2) Chemical catalyst (NaOH) 및 enzyme catalyst (SP435)를 이용한 rTG 합성 ······························································································································ 47
3) HPLC-UVD를 활용한 합성된 rTG의 전환률 분석 ········································· 49
4) 1H-NMR을 이용한 합성된 rTG의 acylglycerol 조성 분석 ····················· 49
5) 합성된 rTG의 TLC를 이용한 TAG molecule의 지방산 조성 분석 ······· 49
6) Chemical catalyst (NaOH 1.0%) 및 enzyme catalyst (SP435 1.0%)의 반응시간별 산가, 과산화물가 변화 측정 ··················································· 50
7) 통계 분석 ····················································································································· 50
Ⅴ. 결과 및 고찰 ····································································································· 52
1. rTG 합성을 위한 scale-up 제조된 고농도 DHA-FAEE 기질 특성 ········· 52
2. 합성된 rTG의 HPLC-UVD를 활용한 반응시간별 전환률 비교 ················· 54
1) NaOH를 이용한 촉매량 (0.5%, 1.0%, 1.5%)에 따른 rTG의 전환률 비교 ·········································································································································· 54
2) SP435를 이용한 효소량 (0.5%, 1.0%, 1.5%)에 따른 rTG 전환률 비교 ··········································································································································· 55
3. 합성된 rTG의 1H-NMR을 이용한 반응시간별 acylglycerol 조성 변화
··················································································································· 59
1) NaOH를 이용한 촉매량 (0.5%, 1.0%, 1.5%)에 따른 rTG의 acylglycerol 조성 변화 ·············································································································· 59
2) SP435를 이용한 효소량 (0.5%, 1.0%, 1.5%)에 따른 rTG의 acylglycerol 조성 변화 ················································································ 61
4. 합성된 rTG의 TLC를 이용한 TAG molecule의 반응시간별 지방산 조성 변화
······················································································································· 64
1) NaOH를 이용한 촉매량 (0.5%, 1.0%, 1.5%)에 따른 TAG molecule의 지방산 조성 변화 ··································································································· 64
2) SP435를 이용한 효소량 (0.5%, 1.0%, 1.5%)에 따른 TAG molecule의 지방산 조성 변화 ··································································································· 68
5. NaOH 1.0% 및 SP435 1.0%의 반응 시간별 산가, 과산화물가 변화 ·········· 72
Ⅵ. 요약 및 결론 ····································································································· 74
참고문헌 ·············································································································· 75
