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감자 중심공동 개선 및 맹아억제제 chlorpropham의 건부병 억제 효과

Improvement of the hollow heart of potato and dry rot suppression of a potato sprout suppressant, chlorpropham

초록/요약 도움말

Physiological disorders occurring during potato cultivation include black heart disease, brown spot, secondary growth, degiscence, and hollow heart. The new potato variety “Yeokgang” is resistant to potato late blight and early blight. However, Yeokgang have hollow heart problem. To improve this problem, two experiments were conducted. First, to determine the effect of planting space on hollow heart on “Yeokgang” potato cultivar, this cultivar was grown at three plant spacings (20, 25, and 30 cm) at highland. Second, the same cultivar was grown at both highland and lowland places and was treated with Boric acid, CaCl2, Boric acid+CaCl2, Sulfur fertilizer, Diniconazole SC, and Prohexadione-calcium SC to find out how these treatments can improve the hollow heart disorder on “Yeokgang”. As a result, according to the first experiment, the incidence of hollow heart disease was 15.7%, 13.7%, and 18.4% at 20, 25, and 30 cm plant spaces, respectively, and was the lowest at 25 cm. According to the second experiment, the ratio of hollow heart in the control was 12.9% and 24.0% in lowland and highland respectively. At the lowland, experiment diniconazole SC treatment as 1.9% reduced the hollow heart disorder better than nontreatmentSas 12.9% whereas at highland, Prohexadione-calcium SC treatment as the ratio of 14.0% reduced the hollow heart disorder better than nontreatments as 24.0%. According to the above results, appropriate potato plant spacing (25cm) and as well as proper uses of Diniconazole SC and Prohexadione-calcium SC can expectedly improvement the incidence of hollow heart disorder of “Yeokgang” potato during cultivation period. Potato dry rot is one of the potato storage diseases caused by Fusarium spp. If sprouts occur during storage of potatoes, the sprouts can be suppressed by treatment with chloropropam, so that the potatoes can be stored for a long period of time. Dry rot caused by Fusarium spp. causes large yield losses during potato storaging. In this study, an experiment was conducted to confirm the inhibitory effect of chlorpropham used to inhibit the appearance of sprouts during storage. Eight Fusarium spp. were isolated from potatoes infected by dry rot in order to obtain pathogenic strains, and among them, the highly pathogenic Fusarium sp. S-3 strain was selected as a test strain. Chlorpropham (5038, 503.8, 50.4, 5.0 ppm) and prochloraz (100, 10, 1, 0.1 ppm) were investigated on inhibition of hyphal growth in pesticide containing media. As a result, the inhibition rates were 100%, 98.2%, 76.0%, 1.8% and 100%, 100%, 98.6%, 53.8% for chlorpropham and prochloraz treatments respectively. The dry rot suppression of chlorpropham (503.8, 50.4, 5.0 ppm) and prochloraz (100 ppm) against F. oxysporum at different concentrations were observed at room temperature (20℃) and low temperature (4℃) and the disease development was monitored. At room temperature, the diseased diameter was 13.2, 10.2, 12.7, 13.2 mm and 12.6, 13.2 mm according to treatments with chlorpropham and prochloraz respectively. In vivo the inhibition of potato dry rot was suppressed compared to other treatments from 50.4 ppm to 10.2 mm based on chlorpropham. However, at low temperature, the diseased diameter was the widest in the control (9.2 mm), but there was no statistical difference between treaments. As a result, postharvest treatment with chlorpropham 50.4 ppm to prevent sprouting and potato dry rot during the storage can be useful.

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목차 도움말

ABSTRACT ⅰ
Contents ⅳ
List of tables vii
List of figures ix

Ⅰ. 서언 1
1. 감자 1
1.1. 감자의 기원 및 영양학적 가치 1
1.2. 감자 국내재배 현황 및 전망 1

2. 감자 중심공동 3

3. 감자 건부병 3

4. Chlorpropham 4
4.1. Chlorpropham 작용기작 4
4.2. Chlorpropham 화학적 구조 4
4.3. Chlorpropham 특징 및 생태 위해성 평가 5

5. 연구배경 및 연구목적 7
5.1. 연구배경 7
5.2. 연구목적 7

Ⅱ. 재료 및 방법 8
1. 중심공동 개선에 대한 실험 처리 기준 설정 8
1.1. 공시품종 설정 8
1.2. 재배 기준 설정 8
1.2.1. 재배기간 및 장소 8
1.2.2. 재식거리 설정 8
1.3. 처리물질 기준 설정 8
1.3.1. 실험기간 및 장소 8
1.3.2. 처리량 및 처리방법 설정 9

2. 처리별 중심공동 개선 효과 검정 11
2.1. 재식거리에 따른 개선 효과 11
2.2. 처리물질에 따른 개선 효과 11
2.3. 조사방법 11

3. F. oxysporum에 대한 chlorpropham의 억제 효과 12
3.1. 공시균주 설정 12
3.2. 공시품종 설정 12
3.3. 병원균 수집 12
3.4. 분리 및 동정 13
3.5. 약제배지 스크리닝 13
3.6. 조사방법 13

4. Chlorpropham의 감자 건부병 방제효과 검정 15
4.1. 경종개요 15
4.2. 약제 희석배수 및 약제처리 15
4.3. 병원균 접종 15
4.4. 약효조사 15

Ⅲ. 결과 및 고찰 18
1. 처리별 중심공동 개선 효과 검정 18

2. F. oxysporum에 대한 chlorpropham의 억제 효과 26
2.1. 분리 및 동정 26
2.2. 약제배지 스크리닝 26
3. Chlorpropham의 감자 건부병 방제효과 검정 33
3.1. 맹아율 33
3.2. 건부병 방제효과 33

Ⅳ. 적요 41
Ⅴ. 참고문헌 43

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