Research on the alleviation of obesity and metabolic syndrome by Gymnaster koraiensis in high-fat diet animal model and its molecular mechanism
고지방식이 동물모델에서 벌개미취 추출물의 비만 및 대사증후군 완화 효과와 이에 대한 분자기전연구
- 주제(키워드) 도움말 "Gymnaster koraiensis" , "Metabolic syndrome" , "obesity" , "AMP-activated protein kinase" , "UCP1-indefendent energy consumption"
- 발행기관 강릉원주대학교 일반대학원
- 발행년도 2020
- 학위수여년월 2020. 8
- 학위명 석사
- 학과 및 전공 도움말 일반대학원 KIST강릉분원학.연협동과정
- 실제URI http://www.dcollection.net/handler/kangnung/000000010745
- UCI I804:42001-000000010745
- 본문언어 영어
초록/요약 도움말
Metabolic syndrome is a medical term that represents a state of co-occurrence of more than three pathologic risk factors like abdominal obesity, insulin resistance, hypertension, hyperglycemia, and hyperlipidemia. Gymnaster koraiensis (GK) is an edible plant of Korea known for its anti-cancer, anti-oxidative, and hepatoprotective properties. In this study, the GK ethanol extract was utilized for treating obesity and metabolic syndrome using 3T3-L1 preadipocyte and dietary induced obese (DIO) mice model. Treatment of GK extract on 3T3-L1 cell effectively inhibited adipogenesis and lipid accumulation. Administration of GK extract to DIO mice induced significant reduction in body weight, weight of fat tissues, and size of white adipose tissues. It also ameliorated cardiovascular disease risk by regulating hyperlipidemia and adiponectin contents. Moreover, GK extract improved insulin resistance by lowering fasting blood glucose levels, mitigating oxidative stress and inflammation. In addition, supplementation of GK extract in DIO mice resulted in elevated energy expenditure in white adipose tissue by increased mitochondrial oxidative capacity through upregulated adenosine monophosphate-activated protein kinase (AMPK) signaling. In conclusion, GK extract treatment prominently ameliorated metabolic syndrome related symptoms like obesity, hyperlipidemia, hyperglycemia, and insulin resistance. Hence, GK extract can be a promising multi-target functional food which can improve metabolic syndrome related symptoms.
more목차 도움말
CONTENTS
LIST OF FIGURES Ⅰ
LIST OF TABLES Ⅱ
ABBREVIATIONS USED Ⅲ
ABSTRACT Ⅴ
1. INTRODUCTION 1
2. MATERIALS AND METHODS 5
2.1. Plant materials and high performance liquid chromatography 5
2.2. Cell culture and adipogenesis induction 6
2.3. Cell viability 7
2.4. Oil red O staining 7
2.5. Animal Experimental Design 8
2.6. OGTT and IPITT test 9
2.7. Histological examination and adipocyte cell size assessment 9
2.8. Enzyme immunosorbent assay and colorimetric assay kit analysis. 10
2.9. Western blot analysis 10
2.10. Quantitative real-time polymerase chain reaction 12
2.11. Statistical analysis 14
3. RESULTS 15
3.1. Preliminary research 15
3.2. GK extract has inhibitory effects on adipogenesis and lipid accumulation in 3T3-L1 cells by decreased adipogenic and lipogenesis related proteins 19
3.3. GK intake reduced weight gain and adipocyte size of eWAT by decreasing adipogenic and lipogenesis related proteins and increasing fatty acid oxidation related protein in DIO mice 23
3.4. GK treatment improved serum biochemical parameters and hormone secretions in DIO mice 28
3.5. GK extract ameliorated oxidative stress and obesity-related inflammation in DIO mice 33
3.6. GK administration suppressed insulin resistance in DIO mice 37
3.7. GK administration increased energy expenditure in eWAT of DIO mice 40
3.8. Identification of major compounds in GK ethanol extract 44
3.9. Major compounds of GK ethanol extract (3,5-DCQA, 4,5-DCQA, and chlorogenic acid) showed less efficacies than GK extract for inhibition of lipid accumulation and adipogenesis and lipogenesis related proteins expressions. 46
4. DISCUSSION 49
5. REFERENCES 55
국 문 요 약 62
