Indocyanine green을 이용한 광열 치료의 다종 우식원성 치면세균막에 대한 효과
Effect of Photothermal Therapy with Indocyanine green in Multispecies Biofilm
- 주제(키워드) 도움말 광역동 치료 , 광열 치료 , Indocyanine green , 유아기 우식증
- 발행기관 강릉원주대학교 일반대학원
- 지도교수 도움말 박호원
- 발행년도 2020
- 학위수여년월 2020. 8
- 학위명 석사
- 학과 및 전공 도움말 일반대학원 치의학과
- 실제URI http://www.dcollection.net/handler/kangnung/000000010741
- UCI I804:42001-000000010741
- 본문언어 한국어
초록/요약 도움말
The purpose of this study is to investigate the antibacterial effects of indocyanine green and near-infrared diode lasers on multispecies biofilms. Multispecies biofilm of Streptococcus mutans, Lactobacillus casei and Candida albicans was treated with photosensitizer ICG and a near-infrared diode laser. Colony forming unit (CFU) was measured, and qualitative evaluation of biofilm was performed with confocal laser scanning microscopy (CLSM). Temperature measurement was conducted to evaluate phothothermal effect. In the groups using ICG and diode laser, a significant reduction in CFU was observed, but the difference in antibacterial effect on L. casei and C. albicans with irradiation time was not significant, and similar results were confirmed with CLSM. Group with ICG and diode laser shows higher temperature elevation than group without ICG, and it’s similar with hyperthermia. In conclusion, ICG and near-infrared diode lasers showed significant antibacterial effects on multispecies biofilm, but studies on protocol are necessary for clinical application.
more목차 도움말
Ⅰ. 서론
Ⅱ. 재료 및 방법
1. 세균 배양
2. 바이오필름 형성
3. 광감각제와 광원
4. 바이오필름 처치
5. Colony-forming unit (CFU) 측정
6. 공초점 레이저 주사 현미경 관찰 (Confocal laser scanning microscopy: CLSM)
7. 바이오필름 표면 온도 측정
8. 통계 분석
Ⅲ. 연구 성적
1. Colony-forming unit (CFU) 측정
2. 공초점 레이저 주사 현미경 관찰 (Confocal laser scanning microscopy: CLSM)
3. 바이오필름 표면 온도 측정
Ⅳ. 총괄 및 고찰
Ⅴ. 결론
Ⅵ. 참고문헌
Ⅶ. 영문초록
