Dictyota coriacea and Agarum cribrosum ethanol extracts inhibit melanin synthesis and collagenase activity in artificial skin
인공피부 내에서 참가죽그물바탕말, 구멍쇠미역 에탄올 추출물의 melanin 생성 억제 및 collagenase 억제 활성
- 주제(키워드) 도움말 Artificial skin , Fontana-Masson stain , Immunohistochemistry stain , anti-wrinkle , inhibition of melanin synthesis
- 발행기관 강릉원주대학교
- 발행년도 2019
- 학위수여년월 2019. 8
- 학위명 석사
- 학과 및 전공 도움말 일반대학원 해양생물공학과
- 실제URI http://www.dcollection.net/handler/kangnung/000000010531
- UCI I804:42001-000000010531
- 본문언어 영어
초록/요약 도움말
With the aim of reducing or replacing animal testing, many in vitro methods have recently been provided as alternatives to using animals. A variety of human skin equivalents have been developed as alternatives to animal testing, and some of them are commercially available. Human skin equivalents can be divided broadly in to two categories. One is an epidermal model containing only normal human keratinocytes cultured to form a multilayered epidermis, such as EpiDerm (MatTek, MA, USA) and EpiSkin (SkinEthic, France). Melanin is produced in melanocytes located in the basal layer of the epidermis of skin, and is a major determinant of skin color. Melanin accumulation in keratinocytes can make skin darker. Although melanin pigmentation in the skin is a protective mechanism against UV radiation, excessive accumulation of melanin induces hyperpigmentation and even pigmentation disorders such as melasma, freckles, and senile lentigines. The synthesized melanin is transferred to keratinocytes by melanocyte dendrites. Melanin accumulation in keratinocytes can make skin darker. Therefore this study aimed at identifying novel cosmetic ingredients as the development of cosmetic products from novel bioresources mushrooming. Edible seaweed has been consumed and used in traditional medicine for centuries by the east ancient people. In the present study, the anti-melanogenic and anti-wrinkle properties of seaweed extracts from Dictyota coriacea and Agarum cribrosum on the artificial skin were aimed to outline. The result of Fontana-Masson stained artificial skin (Neoderm-Me) revealed that the treatment with F1-T-F17 (Dictyota coriacea) and EA-F4-g (Agarum cribrosum) fractions of seaweed extracts significantly decrease the melanin contents by 60%. And IHC (immunohistochemitric) staining of artificial skin (Neoderm-ED) treated with F1-T-F17 and EA-F4-g fractions of seaweed extracts significantly impacted on wrinkle formation. Collectively this study confirms the applicability of Dictyota coriacea and Agarum cribrosum extracts in novel cosmeceutical formulations.
more목차 도움말
Lists of Figures...............................................................................................ⅰ
Abstract...............................................................................................................1
1. Introduction 3
2. Materials and Methods 5
2.1. Material 5
2.2. Melanin contents assay 5
2.3. Fontana-Masson staining 6
2.4. IHC (Immunohistochemstry) staining 6
2.5. NO assay 6
2.6. Western blot 7
2.7. Statistical analysis 7
3. Results and Discussion 8
3.1. Melanin contents assay 8
3.2. Fontana-Masson staining 8
3.3. IHC (Immunohistochemistry) staining 13
3.4. NO assay 18
3.5. Western blot 18
4. Conclusion 24
5. Abstract (in Korean) 25
6. Reference 26
