콩 핵심집단을 활용한 기능성 작물 대사체 플랫폼 연구
Functional Metabolomic Platform Using Soybean Core-Collection
- 주제(키워드) 도움말 soybean , core-collection , antioxidant activity , correlation analysis , functional metabolite platform
- 발행기관 강릉원주대학교 일반대학원
- 발행년도 2019
- 학위수여년월 2019. 8
- 학위명 석사
- 학과 및 전공 도움말 일반대학원 KIST강릉분원학.연협동과정
- 실제URI http://www.dcollection.net/handler/kangnung/000000010472
- UCI I804:42001-000000010472
- 본문언어 영어
초록/요약 도움말
Soybean is a main crop in terms of economic and nutritional aspects, containing rich amounts of protein, starch, essential minerals as well as secondary metabolites. Soybean core collection used in this study consisted of more than 400 soybean resources and well-reflected more than 99% genetic diversity of 26,000 soybean accessions. Using this core-collection, new metabolites and bioactive compounds can be found based on metabolite-bioactivity association study without traditional bioactivity guided fraction, which requires long and repeating fraction procedures. Based on this platform, biological activity of DPPH radical-scavenging assay was performed to obtain IC50 value with six different concentrations. Meanwhile, hepatocyte protection assay was also carried out to find most effective soybean variety. Metabolite profiling data were obtained based on liquid chromatography high- resolution mass spectrometry (LC-HRMS) and more than 100 metabolite features were used for the association study. After obtaining the profiling of metabolite and bioactivity values, data analysis based on correlation analysis was conducted using a Pearson correlation by R program and showed strong association in between 16 metabolites and bioactivities. The results showed that Cyanidin-3-O-glucoside, Peonidin 3-glucoside, and procyanidin B2 had high correlation with biological activity with more than 0.7 correlation constant. Soybeans rich in Cyanidin-3-O-glucoside, Peonidin 3-glucoside, procyanidin B2 and epicatechin showed both high antioxidant activity and high hepatocyte protection activity which were already indicated by the association study. This study demonstrated that functional metabolomic platform by an association study between metabolite and bioactivity based on core-collection can be an efficient way to find bioactive compounds applicable for various bioactivity including as anti-cancer, anti-diabetes, and many others.
more목차 도움말
List of Tables II
List of Figures II
Acknowledgements III
Abstract (Korean) IV
Abstract VI
1. Introduction 1
2. Materials and methods 18
2.1. Chemicals and reagents 18
2.2. Plant materials 18
2.3. Sample preparation 18
2.4. Metabolite analysis 19
2.5. DPPH radical-scavenging activity 20
2.6. Cell viability assay 20
2.7. Data processing and statistical analysis 21
3. Result and discussion 22
3.1. Soybean extraction and DPPH radical-scavenging assay 22
3.2. Cell viability assay 28
3.3. Association between bioactivity and metabolites 32
4.conclusion 50
References 52
