육상오염원이 강릉시 패류생산해역에 미치는 세균학적 영향
Bacteriological Effects of Land Pollutant Sources on the Shellfish Growing Area at Gangneung-si in East Sea, Korea
- 주제(키워드) 도움말 강릉시 패류생산해역 , 육상오염원 , 대장균군 , 분변계대장균 , E. coli
- 발행기관 강릉원주대학교 일반대학원
- 지도교수 도움말 신일식
- 발행년도 2019
- 학위수여년월 2019. 8
- 학위명 석사
- 학과 및 전공 도움말 일반대학원 해양식품공학과
- 원문페이지 78
- 실제URI http://www.dcollection.net/handler/kangnung/000000010457
- UCI I804:42001-000000010457
- 본문언어 한국어
초록/요약 도움말
The production, consumption and exportation of Korean fishery products has increased every year since 1990. For this reason, the shellfish growing area is increasing in Korea. The bacteriological safety of shellfish is very important in the shellfish growing area because the shellfish growing area is often effected by land pollutant sources, domestic water and stream water. This study was carried out to evaluate the effect of land pollutant sources on the seawater and shellfish in shellfish growing area at north sea area and south sea area of Gangneung-si in East sea, Korea by rainfall. The total 43 samples (15 samples of land pollutant sources, 24 samples of seawater and 4 samples of shellfishes) were collected from north sea area (Jumunjin-eup) to south sea area (Okgye-myeon), Gangneung-si in East Sea, Korea. The coliform group, fecal coliform and Escherichia coli in land pollutant sources, seawater and shellfish were analysed. The range of coliform group in land pollutant sources, seawater and shellfish at the day before rainfall were 54~35,000 MPN/100 mL, 6.8~70 MPN/100 mL and 230~390 MPN/100 g respectively. The range of fecal coliform in land pollutant sources, seawater and shellfish at the day before rainfall were 22~6,200 MPN/100 mL, <1.8~34 MPN/100 mL and 62~70 MPN/100 g respectively. The range of E. coli in shellfish at the day before rainfall was 35~54 MPN/100 g. The range of coliform group in land pollutant sources, seawater and shellfish at 1st day after rainfall were 110~54,000 MPN/100 mL, 20~180 MPN/100 mL and 2,200~5,400 MPN/100 g, respectively. The range of fecal coliform in land pollutant sources, seawater and shellfish at 1st day after rainfall were 49~17,000 MPN/100 mL, <1.8~79 MPN/100 mL and 700~1,300 MPN/100 g respectively. The range of E. coli in shellfish at 1st day after rainfall were 160~280 MPN/100 g. The level of coliform group, fecal coliform and E. coli in land pollutant sources, seawater and shellfish were increased sharply at 1st day after rainfall compare to the day before rainfall. And then, slowly decreased at 3rd day after rainfall, and recovered as level before rainfall at 5th day after rainfall. The bacteriological level of seawater and shellfish was affected by level of fecal coliform and radius of half circle in land pollutant sources. Therefore, if the level of fecal coliform and radius of half circle in land pollutant sources increases, the level of fecal coliform of seawater and shellfish increases, too. These results could contribute to bacteriological management of shellfish growing area from land pollutant sources at rainfall time and rainy season.
more목차 도움말
Ⅰ. 서 론 1
Ⅱ. 재료 및 방법 8
1. 조사 대상 해역의 지정학적 특징 8
1.1. 강릉 북부 해역 8
1.2. 강릉 남부 해역 8
2. 시료 11
2.1. 시료 11
2.2. 하천수와 생활하수의 유량 측정 11
2.3. 시료 채취 시기 12
2.4. 시료 채취 장소 14
2.4.1. 강릉 북부 해역 14
(1) 육상오염원 14
(2) 해수 및 패류 14
2.4.2. 강릉 남부 해역 17
(1) 육상오염원 17
(2) 해수 및 패류 17
3. 세균수 측정 20
3.1. 시약 20
3.2. 대장균군, 분변계대장균 및 Escherichia coli의 정량분석 21
(1) 대장균군 21
(2) 분변계대장균 24
(3) Escherichia coli 27
4. 육상오염원 영향평가 29
Ⅲ. 결과 및 고찰 31
1. 강우에 따른 육상오염원의 세균학적 변화 31
1.1. 강릉 북부 해역 육상오염원 31
1.2. 강릉 남부 해역 육상오염원 34
2. 강우에 따른 해수의 세균학적 변화 37
2.1. 강릉 북부 해역 해수 37
2.2. 강릉 남부 해역 해수 40
3. 강우에 따른 패류의 세균학적 변화 43
3.1. 강릉 북부 해역 패류 43
3.2. 강릉 남부 해역 패류 48
4. 해수와 패류에 대한 육상오염원의 영향 52
4.1. 강릉 북부 해역 해수 52
4.2. 강릉 남부 해역 해수 54
4.3. 강릉 북부 해역 패류 56
4.4. 강릉 남부 해역 패류 58
Ⅳ. 결 론 60
Ⅴ. 참고문헌 63
Ⅵ. 감사의 글 66
