수산물 위판장의 수산물과 주변 환경에 대한 미생물학적 오염도 조사
Microbiological Contamination of Fishery Products and Environment in Fishery auction market
- 주제(키워드) 도움말 수산물 , 위판장 , 미생물학적 오염도
- 발행기관 강릉원주대학교 일반대학원
- 지도교수 도움말 신일식
- 심사위원 김희연, 유상권
- 사용가능일 20181231
- 발행년도 2018
- 학위수여년월 2019. 2
- 학위명 석사
- 학과 및 전공 도움말 일반대학원 해양식품공학과
- 실제URI http://www.dcollection.net/handler/kangnung/000000010436
- UCI I804:42001-000000010436
- 본문언어 한국어
초록/요약 도움말
In recent, consumers attention for safety of the fishery products is increasing, but fishery products are not being properly managed at the fishery auction markets from time to time. Fishery auction markets are one of the risk places for microbial contamination of fishery products. If the equipment, seawater and environmental factors of fishery auction market where the fishery product is distributed are contaminated by microorganisms, fishery products may be cross-contaminated by microorganisms. Therefore, this study was carried out to obtain a basic data for establishing a sanitary management of fishery auction markets. Investigation of microbiological (viable cell count, coliform group, Escherichia coli, and pathogenic Vibrio sp.) contamination was carried out in live fish, fresh fish and market environments, and investigation of histamine was also carried out in Yellow tail (Seriola quinqueradiata) at Jumunjin fishery auctionmarket (Gangwon-do, South Korea) at March, June and September, 2017. In case of live fish, 91 MPN/100 g of V. parahaemolyticus was detected from Yellow tail in the storage step whereas, V. parahaemolyticus was not found in preparation and auction steps of Yellow tail. Concerning fresh fish, 3.0×101 CFU/g of E. coli was detected in Sailfin sandfish (Arctoscopus japonicus) of storage step. However, preparation and auction steps of Sailfin sandfish indicated no microbiological contamination of E. coli. E. coli and V. parahaemolyticus were not detected in the preparation and auction steps of fishery products, but were detected in the storage step of fishery products, presumably due to cross-contamination by the microorganisms in the environmental factors of the fishery auction market. Market environmental factors are also important consideration of sanitary processing of fishery products. E. coli with 7.0×101 CFU/100 cm2 was detected from fishery auction market floor collected in June. In addition, 3.0×102 CFU/100 cm2 of E. coli and 91 MPN/100 cm2 of V. parahaemolyticus were detected in surface of auction market floor collected in September. Incase of histamine, 4.3 mg/kg was detected in Yellow tails collected at March. Also, 3.1 and 4.9 mg/kg was detected in September, but below the regulation limit (200 mg/kg, MFDS). Our study proposed that storage step has the highest risk of V. parahaemolyticus and E. coli among preparation, auction and storage steps. This microbiological risk might be attribute to cross contamination from market floor during storage, and suggests the need to improve sanitary and quality managements at fishery auction market.
more목차 도움말
List of tables-ⅰ
List of figures-ⅱ
Abstract-ⅲ
Ⅰ. Introduction- 1
Ⅱ. Materials and Methods-4
1. Materials-4
2. Methods-7
2.1. Measurement of viable cell count-7
2.2. Measurement of coliform group and Escherichia coli-8
2.3. Measurement of pathogenic Vibrio sp.-9
2.3.1. Treatment of sample-9
2.3.2. Identification of pathogenic Vibrio sp.-10
2.4. Measurement of histamine amount-12
Ⅲ. Results and Discussion-13
1. Investigation of microbiological contamination-13
1.1. Live fish-13
1.2. Fresh fish-17
1.3. Environmental factor-21
2. Amount of histamine-25
Ⅳ. Conclusions-27
Ⅴ. References-29
Ⅵ. 감사의 글-35
