배합 사료내 녹색 색소 원료에 따른 참다슬기(Semisulcospira coreana)의 성장 및 가식부 클로로필 함량에 미치는 영향
Dietary supplementation of natural pigments on the growth and chlorophyll content of Semisulcospira coreana
- 주제(키워드) 도움말 참다슬기 , 클로로필 , spirullina , chlorella
- 발행기관 강릉원주대 일반대학원
- 지도교수 도움말 이상민
- 심사위원 홍수희. 김이경, 이상민
- 사용가능일 20181227
- 발행년도 2018
- 학위수여년월 2019. 2
- 학위명 석사
- 학과 및 전공 도움말 일반대학원 해양생명공학과
- 실제URI http://www.dcollection.net/handler/kangnung/000000010392
- UCI I804:42001-000000010392
- 본문언어 한국어
초록/요약 도움말
Semisulcospira coreana is one of the 9 species of melania snails that are one of the most widespread freshwater snails in far east Asia. In Korea, S. coreana have long been used as a seasoned shellfish, with color being one of the most important quality criteria dictating the market value of this freshwater snail. Because S. coreana cannot synthesize pigments de novo, they must obtain these compounds from dietary source to achieve their pigmentation. Given the high costs of synthetic pigments, however, efforts have been made to evaluate the potential of natural compounds. Therefore, three separate feeding trials were performed to evaluate the effects of adding various dietary sources of pigments on growth performance and most particularly pigmentation of S. coreana. In the first trial (EXP. I), 2400 snails were randomly distributed in a closed water recirculated system and fed twelve particulate diets for 12 weeks. Diets were formulated to contain a constant level of pigments (200 g kg−1) from different sources including, Chlorella, Spirulina, green laver, Seaweed fulvescens, synthetic edible dye, green tea, mugwort, Kale, broccoli, kelp, and dried laver. A fishmeal-based diet without dietary inclusion of pigment was considered as a control diet. Results showed that the inclusion of dietary pigments had no significant effect on snail growth performance. However, total chlorophyll and chlorophyll a contents of the edible portion of the snails were markedly affected by dietary inclusion of various pigments and the highest values were found in snails fed on the diets containing Spirulina and green laver compared to those fed on control pigment-free diet. In the second (EXP. II) and third (EXP. III) experiments, the effect of Spirulina and green laver which have been highly effective in the EXP. I and Chlorella which contain high level of chlorophyll were examined in diets for relatively smaller 0.5 cm and bigger 1.0~1.5 cm snails than those examined in the EXP. I. In both experiments, snails were raised for 8 weeks and no statistically significant differences in their growth performance were noted. In EXP. II, supplementing small snail’s diets with Chlorella powder significantly improved total chlorophyll and chlorophyll a contents of their edible portions compared to the other three dietary treatments. In EXP. III, chlorophyll a contents of the edible portions of the relatively bigger snails were also significantly increased by dietary Chlorella supplementation. In addition, significantly higher total chlorophyll levels were found in snails fed on Chlorella and Spirulina diets compared to those fed on control and green laver diets. The present findings, therefore, suggest that feeding the diet containing 200 g kg-1 Spirulina, green laver or Chlorella could improve the body pigmentation of S. coreana without any adverse effects on its growth performance.
more목차 도움말
LIST OF TABLES i
LIST OF FIGURES ii
Abstract 1
1. 서론 7
2. 재료 및 방법 9
2.1.실험사료 9
2.2.실험어 및 사육관리 10
2.3. 어체측정 및 성분분석 16
2.4. 통계처리 17
3. 결과 및 고찰 18
4. 참고문헌 40
