동자꽃을 이용한 항산화 기반 뷰티 소재 개발
Development of Antioxidative Activity-based Beauty Functional Materials Using Lychnis cognata
- 주제(키워드) Lychnis cognata , on-line HPLC-ABTS , DPPH assay , beauty functional material
- 발행기관 강릉원주대학교 일반대학원
- 지도교수 김병섭, 엄병헌
- 발행년도 2018
- 학위수여년월 2018. 2
- 학위명 석사
- 학과 및 전공 일반대학원 KIST강릉분원학.연협동과정
- 본문언어 한국어
- 저작권 강릉원주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Lychnis cognata Maxim(Caryophllaceae) is a flowering plant native to Korea. Lychnis genus contains 25 species in the world and in Korea has 4 species L. cognata, L. fulgens, L. wilfordii, L. kiusiana. Because of its beautiful flower, L. cognata has been used as an art and ornamental material, which even produced three story books and legends about this flower in Korean mountain regions. Until now, Lychnis flowers have been used only for ornamental purposes and not have been developed as beauty materials. Therefore in this study, we studied Lychnis cognata for developing as a beauty functional material. As a basic research for using as a beauty material, optimal extraction and analysis methods were determined by varying sample size(0.1cm, 0.5cm, 2cm), time(10min, 30min, 60min, 90min, 120min), temperature(4℃, 25℃, 50℃) and solvent conditions(100% Water, 25% Ethanol, 50% Ethanol, 75% Ethanol, 100% Ethanol). And in order to compare the contents of major components of plant parts(Root, Leaf, Stem) according to the cultivation period(Odaesan, Nochusan, Wichon-ri) and harvesting time(May, July, December), on-line HPLC-ABTS analysis and DPPH radical scavenging assay were used. The major components of L. cognata root were isolated and identified, which were two phenolic compounds Ferulic acid, Dihydroferulic acid and one alkaloid Tunicoidine D. For more accurate evaluation of antioxidative activity of L. cognata extract, kinetics behavior of the DPPH radical scavenging activity was studied. DPPH radical scavenging activities of major antioxidants and extracts were evaluated by using radical scavenging patterns and IC50 values. The radical scavenging patterns of major antioxidants and L. cognata extracts were classified into fast-kinetics, fast + slow-kinetics, and slow-kinetics. The crude extracts of root and leaf of L. cognata showed fast-kinetic pattern similar to L-ascorbic acid. But Ethyl acetate fraction of L. cognata root extract showed fast+slow-kinetic pattern similar to the ferulic acid’s on DPPH radical scavenging activity, which explain that has high quantity of ferulic acid. In this study, we studied L. cognata, which had been known only as a wild flower and literary material, in order to develop it as an industrial material. Recently, L. cognata root extract was registered as a cosmetic ingredient on international nomenclature of cosmetic ingredients(INCI) name, and this research showed L. cognata as a promising beauty functional material.
more목차
List of Tables
List of Figures
Ⅰ. 서 론 1
Ⅱ. 재료 및 방법 6
1. 실험재료 및 시약 6
1.1. 실험재료 6
1.2. 시약 6
2. 추출 및 분석방법 설정 7
2.1. 시료크기/시간 7
2.2. 온도/용매 7
2.3. HPLC 분석 7
2.4. UV 흡광도 측정 8
2.5. DPPH 라디칼 소거능 측정 8
3. 재배지, 시기 및 부위별 성분변이 연구 8
3.1. 추출물 제조 8
3.2. Online HPLC-ABTS를 이용한 항산화 분석 9
3.3. DPPH 라디칼 소거능 측정 9
4. 동자꽃 뿌리의 지표/활성 성분의 분리 및 구조규명 10
4.1. 추출 및 용매분획 10
4.2. 분리 및 정제 11
4.3. 구조결정 11
5. 항산화 활성 11
5.1. DPPH 라디칼 소거능 측정 11
5.2. DPPH 라디칼 소거능의 동역학적 특성 12
Ⅲ. 결과 및 고찰 13
1. 추출 및 분석방법 설정 13
1.1. 시료크기와 추출시간 13
1.2. 추출온도 및 용매조성 21
2. 재배지와 시기, 부위별 성분변이연구 27
2.1. 재배지 연구 27
2.2. 채취시기 및 부위별 연구 30
3. 동자꽃 뿌리의 지표/활성 성분의 분리 및 구조규명 38
3.1. Compound 1의 구조동정 38
3.2. Compound 2의 구조동정 38
3.3. Compound 3의 구조동정 39
4. 항산화 45
4.1. DPPH 라디칼 소거능의 동역학적 특성 45
Ⅳ. 결론 53
Ⅴ. 참고문헌 56
Abstract 60
