Anti-wrinkle effect of extract from brown alga, Dictyota coriacea
- 주제(키워드) Anti-wrinkle
- 발행기관 강릉원주대학교
- 지도교수 변희국 교수님
- 발행년도 2018
- 학위수여년월 2018. 2
- 학위명 석사
- 학과 및 전공 일반대학원 웰니스바이오산업학과
- 본문언어 영어
- 저작권 강릉원주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Anti-wrinkle activites of Dictyota coriacea, measured by collagenase inhibition and elastase inhibition, was collected at the cost of Sacheonjin beach in Gangneung, Korea. Among them, n-Hexane fraction of Dictyota coriacea extract exhibited activities both collagenase inhibitory and elastase inhibitory effect. The n-Hexane fraction was separated by Sephadex LH-20, TLC and HPLC chromatography. F1-T-F17 and F3-T-F8 evidenced inhibitory effect with an IC50 value of 0.27 mg/mL and 0.057 mg/mL. F1-T-F17 and F3-T-F8 were molecular weight for 1299 Da and 431Da by Q-TOF-MS. Lineweaver-Burk plots suggest that F3-T-F8 plays as a competitive inhibitor against elastase These compounds were evaluated for their inhibitory effects on collagenase and elastase in CCD-986SK fibroblast cell. In this study, we have examined the inhibitory effects of F1-T-F17 and F3-T-F8 isolated from Dictyota coriacea on collagenase and elastase inhibitory activity and viability CCD-986SK fibroblast cell. Therefore, we suggest that F1-T-F17 and F3-T-F8 might prove useful as a inhibitor of collagenase and elastase for anti-wrinkle.
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Contents
page
List of Tables.....................................................................vii
Lists of Figures................................................................viii
Abstract................................................................................1
1. Introduction.....................................................................2
2. Meterials and Methods...................................................7
2-1. Meterials.......................................................................5
2-2. Preparation of ethanolic extract and fractions........5
2-3. Isolation of Compounds.............................................8
2-4. Quantification of total phenol contents....................8
2-5. Elastase inhibitiory assay...........................................8
2-6. Collagenase inhibitiory assay.....................................9
2-7. Cell culture...................................................................9
2-8. Cell viability assay......................................................10
2-9. Western blot analysis................................................10
2-10.Statistical analysis ...................................................11
3. Results and Discussion................................................13
3-1. Total phenolic contents and antioxidant activities of Dictyota coriacea...............................................................13
3-2. Isolation of compounds of EtOH extract and its solvent fractions from Dictyota coriacea ....................................17
3-3. Determination of the inhibition pattern on elastase ............................................................................................21
3-4. Cell viability effects of inhibitory activity fraction by MTT assay..................................................................................24
3-5. Effect of F1-T-F17 and F3-T-F8 on the protein expression of MMPs, and TIMP in CCD-986SK fibroblast cells...................................................................................26
4. Conclusion....................................................................28
5. 국문요약........................................................................30
6. References...................................................................32
7. 감사의 글.......................................................................40
