齒科用 유니트 水管에서 分離된 細菌의 附着 特性 및 消毒劑에 대한 感受性
- 발행기관 강릉원주대학교
- 지도교수 이시영
- 발행년도 2018
- 학위수여년월 2018. 2
- 학위명 박사
- 학과 및 전공 일반대학원 치의학과
- 세부전공 미생물학 및 면역학
- 본문언어 한국어
- 저작권 강릉원주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
The purpose of this study was to investigate the bacterial diversity in water discharged from dental unit waterlines (DUWLs) using pyrosequencing and to verify the biofilm formation, binding, extracellular polysaccharide production, and coaggregation abilities of the bacterial isolates from these DUWLs. In addition, the present study examined the susceptibility of the DUWL isolates to various disinfectants and confirmed the effects of the disinfectants at susceptible concentrations on multispecies DUWL biofilms formed in the laboratory model. The water samples were collected from 15 dental units located in three different dental clinics in Gangneung city, and four of the 15 samples were selected for pyrosequencing analysis. Isolates from 12 of the 15 water samples were tested for their biofilm formation, binding, extracellular polysaccharide production and coaggregation abilities. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), minimum biofilm inhibitory concentration (MBIC), and minimum biofilm eradication concentration (MBEC) of the disinfectants against these isolates were recorded. The multispecies DUWL biofilm formed using the CDC biofilm reactor was treated at MBIC and the current recommended concentration of the disinfectants for determining the disinfection efficiency. A total of 233 bacteria were obtained from the four pyrosequenced water samples. The most frequently found genera were Sphingomonas (25%) and Halomonas (20%). Based on biofilm accumulation, 12 of the 56 isolates from the 15 water samples were classified under strong, moderate, or weak biofilm-producing groups. The binding ability of the isolates belonging to the strong biofilm-producing group was higher than that of isolates from the other groups. Susceptibility of these 12 isolates was lowest to ethanol and highest to cetylpyridinium chloride. Similar to the effect of disinfectants at the currently used concentration for DUWL disinfection, the use of cetylpyridinium chloride at MBIC or at 1/2 of MBIC in combination with chlorhexidine against multispecies DUWL biofilms exhibited high disinfection effect. The results of this study will help to understand the mechanism of biofilm formation in DUWL and provide basic data required for developing effective disinfection methods.
more목차
국문초록 ……………………………………………………………ⅰ
Ⅰ. 서 론 …………………………………………………………… 1
Ⅱ. 연구 재료와 방법 및 결과 …………………………………… 5
1. 치과용 유니트 내 세균의 다양성 평가 ……………………5
2. 치과용 유니트 수관에서 분리한 분리균주의 바이오필름 형성 능력, 부착 특성, 세포외다당류 그리고 응집 반응 평가…15
3. 치과용 유니트 수관에서 분리한 분리균주의 소독제로 사용되는 화학제제에 대한 감수성 검사 ………………………… 32
4. 소독제로 사용되는 화학제제의 실험실 모델에서 형성한 다종 바이오필름에 대한 소독 효과 비교 ……………………… 41
Ⅲ. 고 찰 ………………………………………………………… 47
Ⅳ. 결 론 ………………………………………………………… 55
Ⅴ. 참고문헌 ……………………………………………………… 57
영문초록 ……………………………………………………………68
