한국(2015~2017)에서의 오이 노균병 (Pseudoperonospora cubensis)에 대한 Oxathiapiprolin의 감수성 기준선 설정 및 포장검정
Baseline Sensitivity and field screening for chemical control to Oxathiapiprolin of Cucumber Downy mildew(Pseudoperonospora cubensis) in Korea
- 주제(키워드) Cucumber , Downy mildew , Pseudoperonospora cubensis , Oxathiapiprolin , Baseline sensitivity , Leaf disc bioassay , Field screening
- 발행기관 강릉원주대학교
- 지도교수 김병섭
- 발행년도 2018
- 학위수여년월 2018. 2
- 학위명 석사
- 학과 및 전공 일반대학원 원예학과
- 세부전공 식물병리
- 본문언어 한국어
- 저작권 강릉원주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Cucumber downy mildew caused by the oomycete Pseudoperonospora cubensis is a potentially serious, most prevalent disease in Korea same as distributed throughout the world disease of cucurbit crops in the open field and under cover. Ps. cubensis is an obligate parasite and known to develop resistance to fungicides very rapidly. It binds in the oxysterol-binding protein domain of Oomycetes. This fungicide have technical features such as effective at very low a. i. user rates, systemic movement and redistribution to protect developing leaves, rainfast, flexible application methods, excellent preventive activity and residual disease control and so on. First objective of this study was to find out Baseline sensitivity about Ps. cubensis of the new anti-oomycete compound oxathiapiprolin using through leaf disc bioassay. Second is to confirm the effect of inhibiting spore germination. last, find efficient dilution factors through the field screening. The purpose of this study was to find out baseline sensitivity of the new anti-oomycete compound oxathiapiprolin against Ps. cubensis. In 2015~2017 experiment, we used two oxathiapiprolin formulation, oxathiapiprolin OD (10%) and oxathiapiprolin SC (4%). Dimethomorph WP (25%) was used as a reference. From 1 cucumber growing areas (Gangneung-si), total 5 isolates of Ps. cubensis were collected. The EC50 values for oxathiapiprolin OD ranged from 0.00079 to 0.00120㎍/㎖ respectively. In 2016 experiment, we used two oxathiapiprolin formulations, oxathiapiprolin OD (10%) and oxathiapiprolin SC (4%). From 3 cucumber growing areas (Gangneung-si, Gimpo-si and Yangyang-gun), total 22 isolates of Ps. cubensis were collected. The EC50 values for oxathiapiprolin OD ranged from 0.01208 to 0.01594㎍/㎖, oxathiapiprolin SC ranged from 0.01302 to 0.01468㎍/㎖ respectively. In 2017 experiment, we used three oxathiapiprolin formulations, oxathiapiprolin OD (10%), oxathiapiprolin SC (4%) and dimethomorph WP (25%). From 2 cucumber growing areas (Gimpo-si and Gangneung-si), total 10 isolates of Ps. cubensis were collected. The EC50 values for oxathiapiprolin OD ranged from 0.01280 to 0.01390㎍/㎖, oxathiapiprolin SC ranged from 0.0035 to 0.15253㎍/㎖, dimethomorph WP ranged from 0.13029 to 0.15490 respectively. EC 50 values of two formulations and Ps. cubensis isolates from 3 locations were similar. However, the resistance development of cucumber downy mildew collected at some farms in Gangneung proved to be serious. so it needs one more time for baseline sensitivity for better result. field screening a result, the reference concentration of 4,000, which is similar to 1,000 and 2,000, is appropriate.
more목차
제 1 장 서언 1
1. 오이 1
1.1. 오이의 기원 및 영양학적 가치 1
1.2. 오이의 국내 외 수급동향 및 전망 2
2. 오이 노균병(Pseudoperonospora cubensis) 5
3. Oxathiapiprolin 8
3.1. Oxathiapiprolin 작용기작 8
3.2. Oxathiapiprolin의 화학적 구조 9
3.3. Oxathiapiprolin의 위해성 평가 및 특징 9
4. 연구배경 및 연구 목적 11
4.1. 연구배경 11
4.2. 연구목적 13
제 2 장 재료 및 방법 14
1. 오이 노균병에 대한 oxathiapiprolin에 감수성 기준 설정 14
1.1. Leaf disc bioassay 14
1.1.1. 병원균 수집 14
1.1.2. 접종원 준비 16
1.1.3. Leaf disc 준비 17
1.1.4. Stock solution 제조 및 농도 설정 18
1.1.5. 살균제 처리 22
1.1.6. 병원균 접종 22
1.1.7. 병원균 배양 22
1.1.8. 조사 방법 22
2. Oxathiapiprolin의 대한 유주자낭 발아억제 검정 23
2.1. 유주자낭 발아 억제 효과 23
3. Oxathiapiprolin의 희석배수별 오이 노균병 방제 효과 검정 24
3.1. 경종개요 24
3.2. 약제 희석배수 및 약제처리 25
3.3. 약효조사 27
제 3 장 결과 및 고찰 28
1. 오이 노균병에 대한 oxathiapiprolin에 감수성 기준 설정 28
2. Oxathiapiprolin의 대한 유주포자자낭 발아억제 검정 37
3. Oxathiapiprolin의 희석배수 별 오이 노균병 방제 효과 검정 39
제 4 장 결론 47
참고문헌 50
감사의 글 55
