여러지역에서 채집한 이고들빼기 생육특성 비교와 신선채소로 유통시키기 위한 수확 후 처리
Comparison of Growth Characteristics for Land Races Harvested in Main Grown Areas and Postharvest Treatments to Use as a Fresh Leafy vegetable in Crepidiastrum denticulatum.
- 주제(키워드) 이고들빼기 , 표현형 , 유전체크기 , 수확후생리 , 저장 , 차압예냉 , 세척
- 발행기관 강릉원주대학교 일반대학원
- 지도교수 홍세진, 김형석
- 발행년도 2017
- 학위수여년월 2017. 8
- 학위명 석사
- 학과 및 전공 일반대학원 KIST강릉분원학.연협동과정
- 세부전공 원예학
- 원문페이지 75
- 실제URI http://www.dcollection.net/handler/kangnung/000000009872
- 본문언어 한국어
- 저작권 강릉원주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Crepidiastrm denticulatum (Cd) is a natural medicinal plant that has potential to be utilized as a resource of functional food in pharmaceutical company. So far Cd has not been subjected to conventional breeding program yet, ununifomized genetic and phenotypic characteristics of Cd is one of major problems restricting the utilization of Cd through the large-scale cultivation and food processing. As an initial step of characterization in Cd, seeds of wild ecotypes in 10 locations of Gangwon-do were collected and grown in growth room to be analyzed for germination rates, DNA contents, phenotypes including plant area, branch angle and length, and concentration of chlorogenic acid. Among 10 ecotypes, the highest DNA contents were measured in Jeongseon-gun(JSG) as 1.27 pg that is about doubled size of Eundureung (EDR) and Cheongoksan (CHO). In case of seed germinations, JSG showed relatively lower level of germinations compared to EDR and CHO that displayed the highest germination rates among 10 Cd ecotypes. This result suggests that variations in germination rate may be influenced by DNA contents that comprise ploidy level of Cd. To analyze ecological relationships among three Cd ecotypes, DNA contents and concentration of chlorogenic acid in CHO, EDR and Pheongchang-gun Daehwa-myeon Gaesu-ri (PDG) were subjected to phylogenetic tree. As a result, a tree diagram showed that PDG and CHO were in the same clade while EDR was separated in both DNA contents and concentration of chlorogenic acid. Actually, location of EDR was separated with PDG and CHO on the map. This result indicates that ecological separation may effects on variations in DNA contents and chemical concentrations in Cd ecotypes. Different post-harvest treatments were tested to figure out optimum condition during storage of fresh Cd products. First, changes in fresh weight, firmness, appearance of fresh Cd were analyzed in different storage conditions (0℃, 2℃, 5℃ and room temperature) after 0, 3, 6, 9 and 12 days. As a result, weight loss for 0, 2 and 5℃ storage was appeared to be 2 times lower than its changes at room temperature, and 5℃ seems to be optimum storage condition due to frozen problem observed at 2℃ storage. As a second experiment, washing, washing and dry, and washing and forced-air cooling before storage at 2℃ were tested to identify optimum treatment to maintain quality of fresh Cd for two weeks storage period. Among three different treatments, washing before storage showed the lowest weight loss and decrease of phenolic contents compared to other treatments, washing and dry and washing and forced-air cooling for 12 days storage. It suggests that washing and storage at 2℃ may be the best option to maintain both the quality and functional compound (phenolic) level after harvest of fresh Cd. As a final experiment, sensory evaluation was accomplished by panel test for the comparison of taste (texture and bitterness), appearance (discoloration and wither), and overall preference as an aspect of customer among different post-harvest treatments. Washing before storage resulted in relatively higher evaluation scores in comparison to without treatments. Washing and dry before 2℃ storage did not make any significant differences in appearance scores compared to washing before storage until 3 days storage at 2℃, but it rapidly decreased appearance scores during 6, 9 and 12 days of storage. It seems that washing before storage may be more effective to prevent quality depreciation usually caused by moisture loss and diseases. Our data suggests that moisture management during storage period is a major component determining quality and functionality in Cd. Keyword Crepidiastrum denticulatum, Phenotype, Genome size, Post-harvest, storage, Forced-air cooling, Washing
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Contents
Abstract 1
List of tables 5
List of figures 7
I. 서 론 9
II. 재료 및 방법 13
실험 1. 강원도에서 채집한 이고들빼기의 유전체 크기, 생육특성 그리고 기능성 성분간의 상관관계 13
1. 공시재료 13
2. 유전체 크기 측정 13
3. 표현형 측정 14
4. 기능성 성분 분석 16
실험 2. 이고들빼기의 신선채소 유통기간 연장을 위한 수확 후 관리와 저장 온도조건 17
1. 공시재료 17
2. 수확 후 처리조건 17
3. 수확 후 처리 품질조사 18
4. 총 페놀 함량 분석 18
III. 결과 및 고찰 20
실험 1. 강원도에서 채집한 이고들빼기의 유전체 크기, 생육특성 그리고 기능성 성분 간의 상관관계 20
1. 10가지 유전자원의 유전체 크기와 발아율 상관관계 20
2. 유전자원 위치정보에 따른 3종류의 유전체 크기, 생육특성 그리고 기능성 성분의 차이 26
실험 2. 이고들빼기의 신선채소 유통기간 연장을 위한 수확 후 관리와 저장 온도조건 35
1. 수확 후 관리∙조건에 따른 상품성의 차이 35
2. 수확 후 관리∙조건에 따른 총 페놀 함량의 변화 48
3. 수확 후 관리∙조건에 따른 관능평가 비교 53
IV. 적 요 61
V. 참고문헌 65
