다파장 LED광이 적무 및 홍염무 새싹의 생육 및 기능성 물질, 항산화 활성에 미치는 영향과 적무 새싹의 저장 조건 최적화
Effects of Light-emitting Diodes (LEDs) on Growth, Functional Materials and Antioxidant activities of Red Radish and Reddish Radish Sprouts, and Optimization of Storage Conditions of Red Radish Sprout
- 주제(키워드) radish sprouts , glucosinolate , antioxidant , LED light , UV-C , microbial contamination , postharvest treatment
- 발행기관 강릉원주대학교 일반대학원
- 지도교수 홍세진, 김형석
- 발행년도 2017
- 학위수여년월 2017. 8
- 학위명 석사
- 학과 및 전공 일반대학원 KIST강릉분원학.연협동과정
- 세부전공 원예학
- 실제URI http://www.dcollection.net/handler/kangnung/000000009871
- 본문언어 한국어
- 저작권 강릉원주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Young sprout has been received lots of intention due to its enhanced human health promoting bioactivity compared to mature leaf and fruit. In the U.S., about 30% of vegetables are consumed as sprouts. In spite of the importance of the sprout in an industrial manner, effective cultivation methods, especially effect of light quality during short-term cultivation period on growth and quality in sprouts, have not been fully investigated yet. So this study was performed to analyze the growth, contained content of glucosinolate(GS), total anthocyanin, total phenolic, total flavonoid, and antioxidant activity of Red radish and Reddish radish sprouts in single wavelength with LED light sources and multi-wavelengths intended combination of LED light sources. As a second experiment, we investigated how sprout qualities are changed during different storage conditions, and to figure out optimum washing and sterilization treatments to avoid contamination of sprouts The LED single wavelength with LED light sources used to sprout cultivation as light sources were UV, blue, green, red and IR, respectively. As an different treatment, red radish and reddish radish sprouts were subjected to the mixed wavelength that the UV, blue, green, red, and IR were added to red and blue light, respectively. Fluorescent lamp and blue-red-white (BRW) LED were used as controls. As a cultivation condition, the light amount was adjusted to 75 μmol m-2 s-1. Day (light emission) and night (dark condition) period were 14 and 10 hours, respectively. The daytime temperature was 25 ° C, and the night time temperature was 18 ° C. Except for the light wavelength, the other conditions were the same. In the single wavelength with LED light sources comparison, hypocotyl length and fresh weight of Red radish sprout appeared to be increased under the red light(5.2 cm/0.17 g) and IR(5.6 cm/0.15 g) compared to the control of fluorescent light(4.2 cm/0.09 g) and RBW LED(4.5 cm/0.13 g). On the other hand, the total anthocyanin content was significantly increased under the blue light(1.91 %) compared to the control of fluorescent light(1.47 %) and RBW LED(1.88 %). The total flavonoid contents also significantly increased under the blue light(4.41 mg) compared to control of fluorescent lamp(3.75 mg) and RBW LED(2.35 mg) , and total phenolic content was significantly higher in the blue light(55.78 mg) and IR(54.77 mg) treatment compared to control of fluorescent lamp(50.52 mg) and RBW LED(48.62 mg). ABTS radical scavenging activity was significantly higher in the blue light(139.29) and IR(139.30) treatment compared to control of fluorescent lamp(158.39) and RBW LED(147.28), and DPPH radical scavenging activity was significantly higher in the red light(150.53) and green light(176.97), IR(181.03) treatment compared to control of fluorescent lamp(223.48) and RBW LED(218.97). GSs identified by HPLC analysis in Red radish sprout were aliphatic(glucoerucin), aromatic(gluconasturtiin) glucosinolates. Total glucosinolate concentration was significantly increased under the blue light(258.73 mM/g) compared to the control of fluorescent lamp(127.41 mM/g) and RBW LED(84.95 mM/g). As the result of second experimental topic, optimum storage condition and forced-air cooling time were investigated in Red radish sprouts. The optimum storage temperature and forced-air cooling time minimizing loss of sprout qualities including functional compound concentrations were 10 ℃ and 30 minutes, respectively. To avoid bacterial contamination in sprouts during cultivation, effect of sterilization of water using UV-C light in germination container were tested. As a result, UV-C sterilization of container water during 5 days decreased the contamination rate in sprouts, the non-coliform count (3.7 x 106 CFU/g), compared to the sprouts grown in water container without UV-C sterilization, the non-coliform count (3 x 107 CFU/g). However, the total aerobic count was ineffective.
more목차
Abstract ⅰ
List of Tables ⅵ
List of Figures ⅶ
Ⅰ. 서론 1
Ⅱ. 재료 및 방법 5
1. 공시재료 5
2. 시약 및 기구 5
3. 새싹 재배 조건 6
3.1 광원에 따른 적무 및 홍염무 새싹의 재배 조건 6
3.2 수확 후 저장조건 최적화를 위한 적무 새싹의 재배 조건 7
4. 기능성 물질 분석 및 항산화 활성 측정 8
4.1 glucosinolate 함량 분석 8
4.2 Quinone reductase (QR) 활성 측정 8
4.3 총 페놀 함량 측정 9
4.4 총 플라보노이드 함량 측정 9
4.5 총 안토시아닌 함량 측정 10
4.6 항산화 활성 측정 10
4.7 HPLC 분석 11
4.8 통계처리 11
5. 저온 저장 및 차압예냉 처리 조건 11
6. 수확 후 저장조건에 따른 품질 조사 12
7. 세척 및 살균 처리 조건 13
8. 미생물 분석 13
Ⅲ. 결과 및 고찰 14
1. 광원에 따른 적무 및 홍염무 새싹의 생육특성 14
2. 광원에 따른 기능성 물질 및 항산화 활성 20
2.1 glucosinolate 함량 20
2.2 Quinone reductase (QR) 활성 28
2.3 총 페놀 함량 30
2.4 총 플라보노이드 함량 30
2.5 총 안토시아닌 함량 31
2.6 항산화 활성 33
3. 저장 온도에 따른 품질 및 기능성 물질 35
3.1 품질 변화 35
3.2 기능성 물질 39
4. 차압예냉 39
5. 미생물 분석 42
5.1 세척 방법에 따른 미생물 분석 42
5.2 살균 처리에 따른 미생물 분석 44
Ⅳ. 적요 46
Ⅴ. 인용문헌 49
