민들레 부산물 함유 배지에서 재배한 느타리버섯 추출물의 항염증 효과
- 발행기관 강릉원주대학교
- 지도교수 김대원, 박순호
- 발행년도 2017
- 학위수여년월 2017. 8
- 학위명 석사
- 학과 및 전공 일반대학원 치의학과
- 세부전공 생화학 및 분자생물학
- 실제URI http://www.dcollection.net/handler/kangnung/000000009812
- 본문언어 한국어
- 저작권 강릉원주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
The aim of the study is to enhance the physiological activity of oyster mushroom, a food material commonly used throughout the world. For this, the physiological activity of oyster mushroom cultivated on a medium containing dandelion extract residue(ODE), which is well known for its anti - inflammatory effect, was confirmed. To examine the physiological activity of the ODE, hot - water extracts were prepared. The cytotoxicity of the extracts was confirmed with RAW264.7 cells, L929 cells, and was not toxic up to a concentration of 100μg/ml. The anti-inflammatory effect of the ODE extract was confirmed by measuring changes in inflammation-related factors by treating LPS known as a typical pro-inflammatory agent. The measured inflammation-related factors were NO, COX-2, iNOS, TNF-α and IL-1β. It was confirmed that the NO produced in the cells treated with the ODE decreased depending on the treatment concentration. RT-PCR and western blotting were performed to confirm changes in COX-2, iNOS, TNF-α and IL-1β. As a result, it was confirmed that it decreased depending on the treatment concentration of ODE. Based on these results, it was confirmed that the anti - inflammatory effect was increased in the oyster mushroom cultivated in the medium containing dandelion extract residue.
more목차
I. 서론
II. 재료 및 방법
1. 재료
2. 느타리버섯 추출물 제조
3. 세포배양
4. MTT assay 를 이용한 세포독성 측정
5. Nitric oxide (NO) 생성 억제
6. 신호전달인자 확인을 위한 RT-PCR
7. 신호전달인자 확인을 위한 Western Blot
8. 통계분석
III. 실험 결과
1. MTT assay를 통한 세포독성 실험
2. Nitric oxide (NO) 생성 억제
3. RT-PCR
4. Western Blot
IV. 결론 및 고찰
참고문헌
영문초록
