국립강릉원주대학교

초록/요약 도움말

This study was conducted to select the optimum feed raw materials for growth, assimilation efficiency and digestive physiology of sea cucumber juveniles reared in the laboratory. In the experiment of small sea cucumber under 1g in the range of 0.12~0.22g wet weight/ind. reared for 2 months, final wet weight was the highest value of 1.39g/ind. fed main berth feed and the lowest value of 0.24g/ind fed mud feed. Weight gain showed also the highest value of 772.2% fed main berth and the lowest value of 126.3% fed mud feed. Survival showed the highest value of 25.0% fed bathe feed and the lowest value of 6.7% fed mud feed. Specific growth rate showed the highest value of 2.53%/day fed Spirulina sp. feed and the lowest value of 0.50%/day fed mud feed. In the experiment of large sea cucumber more 1g in the range of 0.48~0.72g wet weight/ ind reared for 2 months, final wet weight was the highest value of 3.4g/ind fed main berth and the lowest value of 0.6g/ind. fed soybean meal feed. Weight gain showed the highest value of 485.7% fed main berth feed and the lowest value of 111.1% fed soybean meal feed. Survival showed the highest value of 63.3% fed Sargassum sp. feed and the lowest value of 3.3% fed main berth feed. Specific growth rate showed the highest value of 1.91%/day fed main berth feed and the lowest value of 0.13%/day fed soybean meal feed. In the experiment of sea cucumber more 1g in the range of 0.48~0.84g wet weight/ind reard for 2 months, final wet was the highest value of 2.70g/ind fed Sargassum sp. feed and the lowest value of 1.30g/ind fed Spirulina sp. feed. Weight gain showed the highest value of 203.1% fed Sargassum sp. feed and the lowest value of 54.0% fed Spirulina sp. feed. Survival showed the highest of 87.8% fed Spirulina sp. feed and the lowest value of 65.6% fed beer yeast feed. Specific growth rate showed the highest value of 0.04%/day fed Sargassum sp. feed and the lowest value of 0.01%/day fed Spirulina sp. feed. Assimilation efficiencies of the sea cucumber juveniles under 1g fed bathe, soybean meal, beer yeast, fish meal and control feeds showed 52.7%, 53.8%, 53.2%, 54.5% and 55.4%, respectively, But sea cucumber juvenile fed mud feed showed the lowest value of 34.6%. Assimilation efficiency of the sea cucumber juvenile over 1g feed showed the highest value of 53.9% fed fish meal and the lowest value of 34.5% fed Spirulina sp. feed. Activity of amylase for the sea cucumber juvenile under 1g showed the highest value of 3.3U/mg protein fed bathe feed and the lowest value of 0.4U/mg protein fed mud and control feed. Activity of trypsin showed relatively high values of 32.8%, 30.9 and 21.3U/mg protein fed bathe, kelp and Sargassum sp. feed, respectively, but, very low values of 1.3, 1.1 and 1.3U/mg protein fed beer yeast, main berth and Spirulina sp. feed, respectively. Activity of chymotrypsin showed high value of 40.4U/mg protein, but showed low values of 2.5 and 1.6U/mg protein fed Sargassum sp. and soybean meal feed, respectively. Activity of TG-lipase showed the highest value of 130.3U/mg protein fed fish meal feed and the lowest value of 18.9U/mg protein fed control feed. RNA/DNA ratio in the muscle showed the highest value of 2.09 fed Sargassum sp feed and the lowest value of 1.29 fed kelp feed. Activity of ALPase in the muscle showed the highest value of 1.70 U/mg protein fed Sargassum. sp. feed and the lowest value of 0.39 U/mg protein fed kelp feed. Activity of amylase showed the highest value of 0.54U/mg protein fed main berth feed and the lowest value of 0.03U/mg protein. Activity of trypsin showed the highest value of 10.9U/mg protein fed main berth feed and the lowest value of 0.5U/mg protein fed Spirulina sp. feed. RNA/DNA ratio showed the highest value of 10.5 fed kelp and Sargassum sp. feed and the lowest value of 1.6 fed fish meal feed. It is concluded that the assimilation efficiencies and digestive enzyme activities of each feed material derived from the results of this study will be very useful for selecting the optimal feed source for sea cucumber juveniles.