Chlorhexidine과 Cetylpyridinium chloride에 대한 구강연쇄구균의 감수성에 대한 연구
Susceptibility of Oral Streptococci to Chlorhexidine and Cetylpyridinium chloride
- 주제(키워드) 도움말 viridans streptococci , planktonic , biofilm , chlorhexidine , cetylpyridinium chloride , susceptibility , minimum inhibitory concentration , minimum bactericidal concentration , minimum biofilm inhibitory concentration , minimum biofilm eradication concentration
- 발행기관 강릉원주대학교 일반대학원
- 지도교수 도움말 이시영
- 발행년도 2017
- 학위수여년월 2017. 2
- 학위명 석사
- 학과 및 전공 도움말 일반대학원 치의학과
- 실제URI http://www.dcollection.net/handler/kangnung/000000009219
- 본문언어 한국어
초록/요약 도움말
Objectives: Most bacteria frequently found in the supra-gingival plaque are gram-positive streptococci. It is necessary to remove plaque to reduce gingivitis, periodontal diseases, and dental caries. In many studies, chlorhexidine and cetylpyridinium chloride have been reported to be effective in inhibiting oral bacteria. However, no extensive investigation has been conducted into the susceptibility of oral streptococci to chlorhexidine and cetylpyridinium chloride. Therefore, in this study, we investigated the susceptibility of 80 streptococcal species in planktonic or biofilm states to chlorhexidine and cetylpyridinium chloride. Methods: The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of planktonic streptococci were measured using the micro-dilution method according to the Clinical and Laboratory Standards Institute (CLSI) recommended standards. The minimum biofilm inhibitory concentration (MBIC) and minimum biofilm eradication concentration (MBEC) were also measured using the micro-dilution method, after the streptococci formed biofilms on 96-well plates. Results: In all species, the MIC, MBC, MBIC, and MBEC values were higher for chlorhexidine than for cetylpyridinium chloride. Sensitivity values varied according to bacterial species. For chlorhexidine, the MIC against S. mutans and MBC against S. salivarius were significantly lower than those against other species. However, there was no significant difference among species in susceptibility to chlorhexidine after biofilm formation. Additionally, the values for cetylpyridinium chloride showed no significant difference among bacterial species in either the planktonic or biofilm state. When the susceptibility was assessed using combinations of two bacterial species that formed a biofilm, most combinations followed the high susceptibility values observed for individual bacteria; however, some showed varying results, such as values that were either lower or higher than those for individual bacteria. Conclusions: The susceptibility values for chlorhexidine were higher in all bacterial species than those for cetylpyridinium chloride. Most susceptibility values were higher for biofilms than for planktonic bacteria, although those for some bacterial species were the same. When two bacterial species were tested in combinations, most of the susceptibility values were high; however, variations were also present, such as low values, higher or lower values.
more목차 도움말
Ⅰ. 서론..........................................................................................1
Ⅱ. 연구 재료 및 방법...................................................................5
1. 세균
2. 배양조건
3. 항미생물제
4. 항미생물제의 최소억제농도(MIC) 및 최소사멸농도(MBC)의 측정
5. 항미생물제의 최소 바이오필름 억제농도(MBIC) 및 최소 바이오필름 박멸농도(MBEC) 측정
6. 혼합 세균 선별 및 MIC, MBC, MBIC, MBEC 측정
7. 바이오필름 형성 양 및 세균 수 측정
8. 통계 분석
Ⅲ. 연구 결과................................................................................14
1. 항미생물제의 최소억제농도(MIC) 및 최소사멸농도(MBC) 결과
2. 항미생물제의 최소 바이오필름 억제농도(MBIC) 및 최소 바이오필름 박멸농도(MBEC) 결과
3. 혼합 세균에 대한 항 미생물제의 MIC, MBC, MBIC, MBEC 결과
4. 바이오필름 형성 양 및 세균 수가 감수성 결과에 미치는 영향
Ⅳ. 고찰......................................................................................33
Ⅴ. 결론.......................................................................................39
Ⅵ. 참고문헌................................................................................40
Ⅶ. 영문초록.......................................................................................................49
