검색 상세

인삼으로부터 유래된 Ginsenoside Re와 Ginsenoside Re/Leucine 혼합물의 열처리 후 생리활성효과

  • 한임호 (강릉원주대학교 일반대학원)

초록/요약 도움말

Ginsenoside Re is a triol type triterpene glycoside and is abundantly present in ginseng berry. Ginsenoside Re can be transformed into less-polar ginsenosides, namely, Rg2, Rg6, and F4 by heat-processing. The products of heat-processed Ginsenoside Re inhibited phosphorylation of CDK2 at Thr160 by upregulation of p21 level, resulting in S phase arrest. The products of heat-processed Ginsenoside Re also activated Caspase-8, Caspase-9, and Caspase-3, followed by cleavage of PARP, a substrate of Caspase-3, in a dose-dependent manner. Concurrently, alteration of mitochondrial factors such as Bcl-2 and Bax was also observed. Moreover, pretreatment with Z-VAD-fmk abrogated Caspase-8, Caspase-9, and Caspase-3 activations by the products of heat-processed Ginsenoside Re. We further confirmed that the anticancer effects of the products of heat-processed Ginsenoside Re in AGS cells are mainly mediated via generation of less-polar Ginsenosides Rg6 and F4. The structural change of Ginsenoside and the generation of Maillard reaction products (MRPs) are important to the increase in the biological activities of Panax ginseng. This study was carried out to identify the renoprotective component of P. ginseng using the Maillard reaction model experiment with Ginsenoside Re and leucine. Ginsenoside Re was gradually converted into less-polar Ginsenosides Rg2, Rg6 and F4 by heat-processing, followed by separation of the glucosyl moiety at carbon-20. The free radical-scavenging activity of the Ginsenoside Re–Leucine mixture was increased by heat-processing. The improved free radical-scavenging activity by heat-processing was mediated by the generation of MRPs from the reaction of Glucose and Leucine. The cisplatin-induced LLC-PK1 renal cell damage was also significantly reduced by treatment with MRPs. Moreover, the heat-processed Glucose–Leucine mixture (major MRPs from the Ginsenoside Re–Leucine mixture) showed protective effects against cisplatin-induced oxidative renal damage in rats through the inhibition of Caspase-3 activation.

more

목차 도움말

목 차
Summary ………………………………………………………ⅰ
Ⅰ. 서론 ………………………………………………………1
1. 인삼이야기 …………………………………………………1
1. 1. 인삼의 유래 ……………………………………………1
1. 2. 인삼의 분류 ……………………………………………2
1. 3. 인삼의 약리학적 효능 …………………………………2
1. 4. 열처리 인삼의 성분변화 ………………………………3
2. Ginsenoside 이야기 ………………………………………5
2. 1. 인삼 Ginsenoside ……………………………………5
2. 2. Ginsenoside 구조와 종류 ……………………………6
2. 3. Ginsenoside 구조에 따른 약리학적 효능 …………10
Ⅱ. 재료 및 실험방법 ………………………………………13
1. 재료 및 시약 ………………………………………………13
2. Ginsenoside Re-Leucine 혼합물의 열처리 ……………15
3. Ginsenoside 분석 …………………………………………15
4. 갈변화 화합물 측정 ………………………………………16
5. DPPH radical 소거능력 측정 ……………………………16
6. 총 폴리페놀 함량 분석 …………………………………17
7. 신장세포에서 산화적 손상에 의한 신장보호효과 ……18
7. 1. 신장세포 배양 …………………………………………18
7. 2. 신장세포 보호효과 ……………………………………19
8. 쥐에서 Cisplatin 유발 산화적인 손상에 의한 신장보호효과 ………………………………………………………………20
9. 조직학적 검사 ……………………………………………21
10. 쥐 신장조직의 Western blot 분석 ……………………22
11. 위암세포의 증식억제 효과 ……………………………23
11. 1. 위암세포 배양 ………………………………………23
11. 2. 위암세포 증식억제능력 측정 ………………………24
12. Hoechst staining ………………………………………25
13. Flow cytometric assay …………………………………26
14. 위암세포의 Western blot 분석 ………………………27
15. Caspase activity assay ………………………………28
Ⅲ. 결과 및 고찰 ……………………………………………29
1. Ginsenoside Re󰠚Leucine의 특성연구 ……………………29
1. 1. Ginsenoside Re󰠚Leucine의 열처리 전과 후 분석 …29
1. 2. Ginsenoside Re의 열처리 전과 후 …………………32
1. 3. Ginsenoside Re의 열처리 후 구조변화 ……………35
1. 4. 갈변화 화합물 측정 …………………………………36
1. 5. DPPH radical 소거능력 측정 ………………………38
1. 6. 총 폴리페놀 함량 분석 ………………………………40
2. Ginsenoside Re󰠚Leucine의 신장에 대한 보호효과 …42
2. 1. 신장세포에서 산화적인 손상에 대한 신장보호효과…42
2. 2. 동물실험에서 Cisplatin으로 유도된 산화적인 손상에 대한 신장보호효과 ……………………………………………44
2. 3. 조직학적 검사 …………………………………………49
2. 4. 쥐 신장조직의 Western blot 측정 …………………51
3. Ginsenoside Re󰠚Leucine의 항암효과 …………………53
3. 1. 위암세포의 증식억제 효과 …………………………53
3. 2. Hoechst staining ……………………………………59
3. 3. Flow Cytometric Assay & Western blot……………61
3. 3. 1. Cell cycle arrest ………………………………61
3. 3. 2. Apoptosis …………………………………………65
3. 4. Caspase activity assay ……………………………70
Ⅳ. 결론 ………………………………………………………76
Ⅴ.참고문헌 ……………………………………………………79
감사의 글 ………………………………………………………96

more
반출 Meta View 목록