흰쥐의 우식모델에서 erythrosine을 이용한 photodynamic therapy의 항균 및 탈회 억제 효과
Antimicrobial effect and inhibition of enamel demineralization of photodynamic therapy using erythrosine in rat caries model
- 주제(키워드) 도움말 antimicrobial , chlorhexidine(CHX) , erythrosine , photodynamic therapy(PDT) , rat caries model , Streptococcus mutans
- 발행기관 강릉원주대학교 일반대학원
- 지도교수 도움말 마득상, 유기연
- 발행년도 2016
- 학위수여년월 2016. 8
- 학위명 박사
- 학과 및 전공 도움말 일반대학원 치의학과
- 실제URI http://www.dcollection.net/handler/kangnung/000000008468
- 본문언어 한국어
초록/요약 도움말
Antimicrobial effect and inhibition of enamel demineralization of photodynamic therapy using erythrosine in rat caries model Min Sun Lee (Directed by professor Deuk Sang Ma · Ki Yeon Yoo) Department of Preventive and public health dentistry, Graduate school, Gangneung-Wonju National University Key words : antimicrobial, chlorhexidine(CHX), erythrosine, photodynamic therapy(PDT), rat caries model, Streptococcus mutans Objective : The aim of this study was to determine the antimicrobial effectiveness and impact on preventing demineralization of the tooth for dental caries prevention using photodynamic therapy(PDT) applied to erythrosine in rat caries model. Methods : The experimental group were classified as follows: Ⅰ. PDT groups(N=7) PDT group were irradiated green LED(wavelength range: 520-530 nm) during 2 minutes after 5 μM erythrosine 200 ㎕ application; Ⅱ. CHX group(N=7) were applicated 0.2% CHX; Ⅲ. No Tx group(N=7) didn’t taken treatment, and these tree groups were performed using a rat caries model that was inoculated S. mutans three times using 200 ㎕, 108 CFU/ml S. mutans suspension and cariogenic diet beginning; Ⅳ. SPF group(N=3) didn’t taken treatment and cariogenic diet. PDT group was irradiated green LED(wavelength range : 520-530 nm) during 2 minutes after 5 μM erythrosine 200 ㎕ application and CHX group was applied 0.2% CHX solution. The animals were sacrificed at 33 days old. After right and left first molars extraction in mandible of rat, it shook the tube containing the teeth on vortex, and then the bacterial suspensions were gotten. The bacterial suspensions were diluted to 1/10 ~ 1/10000 concentration, and were plated on blood agar and mitis salivarius-bacitracin(MSB) agar. After that, plates were cultured during 72 hours in incubator providing 5% CO2, 37℃. The CFU/ml was counted by automatic colony counter. To measure enamel mineral condition, tooth were made to the appropriate specimens. Specimens were measured as Knoop hardness number(KHN) and pictures taken by QLF-D were analyzed average of fluorescence loss(∆F) and maximum of fluorescence loss(∆Fmax). The results were analyzed using Kruskal-Wallis test and Mann-Whitney U test at a significance level of 0.05(PSWA 18.0, SPSS Inc., USA) Results : There were significant differences between all groups about CFUs/ml on blood agar plates, CFUs/ml appeared the least in PDT group and CHX and SPF group were followed, most bacteria colonies were formed in No Tx group(P<0.001). Also, there were significant differences between all groups about CFUs/ml on MSB agar plates(P<0.001), PDT group and SPF group showed fewer bacterial colonies than in CHX group and control group, CHX group had found even less than in control group but there were not statistically significant(P=0.581). There were statistically significant difference in the analysis of the KHN values(P<0.001). Depending on the post-test results, SPF group was the highest than all groups and PDT group was higher than No Tx group in KHN values that were statistically significant(P=0.004). There were significant differences in ∆F and ∆Fmax (P=0.001, <0.001). Although PDT group showed significantly less ∆F compared to No Tx group(P = 0.009), a significant difference between the PDT group and CHX groups was not appeared(P = 0.068) and there were no significant differences between CHX group and No Tx group(P=0.312). ∆Fmax values of PDT group were statistically significantly less than CHX group and No Tx group(P=0.013, 0.011), but there were no significant differences between CHX group and No Tx group(P=0.836). Conclusion : This study identified that PDT applied erythrosine reduced bacterial colonization and enamel demineralization in rat caries model. In the future, through clinical trials, Through a future clinical trials we need to determine the applicability of PDT applied erythrosine in clinical conditions.
more목차 도움말
목 차
Ⅰ. 서론 ············································································1
Ⅱ. 연구대상 및 방법 ······························································5
1. 실험재료 및 대상 ··················································································5
1.1. 표준 균주 및 접종 ·············································································5
1.2. 실험동물 ······················································································5
1.3. 광역학치료의 광원과 광감작제 ·····························································6
2. 연구방법 ··················································································7
2.1. 흰쥐의 우식모델에서 구강 내 치아우식증 유도 ········································8
2.2. 군 분류 ······························································································8
2.3. PDT와 CHX의 적용··············································································9
2.4. 미생물학적 분석 ·········································································10
2.4. 치아의 탈회정도 분석 ···································································10
(1) 법랑질의 미세경도 측정 ·······································································10
(2) 무기질 소실량 분석············································································12
3. 통계분석 ··················································································13
Ⅲ. 연구성적 ······································································14
1. 구강 세균 집락수(CFUs/ml) 측정 결과························································14
2. 법랑질의 탈회 ··········································································15
2.1. 법랑질의 미세경도 측정 결과 ···························································15
2.2. 교합면의 형광소실도 측정 결과 ··························································16
Ⅳ. 고안 ···········································································17
Ⅴ. 결론 ···········································································22
Ⅵ. 참고문헌 ······································································23
Ⅵ. 영문초록 ······································································28
