실험실 일반 장비를 이용한 치과용 유니트 수관의 실험실 모델 확립
Establishment of the Dental Unit Waterline Laboratory Model Using General Laboratory Equipments
- 주제(키워드) 도움말 Dental disinfectants , Dental infection control , Laboratory model
- 발행기관 강릉원주대학교 일반대학원
- 지도교수 도움말 이시영
- 발행년도 2015
- 학위수여년월 2016. 2
- 학위명 석사
- 학과 및 전공 도움말 일반대학원 치의학과
- 실제URI http://www.dcollection.net/handler/kangnung/000000008150
- 본문언어 한국어
초록/요약 도움말
The purpose of this study was to establish a simple laboratory model for reproducing dental unit waterlines (DUWLs) biofilms. The bacteria obtained from DUWLs were cultured into an R2A liquid medium for 10 days, following which the culture was stored at -70℃. This stock was then inoculated into the R2A liquid medium and incubated in batch mode. After culture for 5 days, it was inoculated into biofilm formation models containing polyurethane tubing pieces and R2A liquid medium. The biofilm formation was carried out in a well plate model, a CDC biofilm reactor model, and our simple laboratory model. After the biofilm formed for four days in each model, biofilm accumulation, thickness, morphological characteristics and distribution of the composing bacteria were compared. Biofilm accumulation was significantly higher in the well plate model. Biofilm accumulation in the simple laboratory model and the CDC biofilm reactor model was similar. The biofilm within polyurethane tubing was distributed widely and thinly in the well plate model. In the simple laboratory model and the CDC biofilm reactor model, thick bacterial lumps were observed in some parts. Therefore, the simple laboratory model was selected as the final model for testing DUWL disinfection. The effectiveness of disinfectants such as sodium hypochlorite, hydrogen peroxide and chlorhexidine was tested by application to the biofilm formed in the simple laboratory model. Because the lower concentrations of disinfectants were less effective in the reducing the count of bacteria constituting the biofilm of the simple laboratory model is appropriate for comparison of disinfectant effects. This model is expected to be used for experiments for various purposes in further studies.
more목차 도움말
Ⅰ. 서론 1
Ⅱ. 연구 재료 및 방법. 4
1.DUWL 세균 수집 및 보관 4
2.DUWL 모델에 접종을 위한 세균 배양 4
3.DUWL 모델 7
4.바이오필름 분석 10
5.소독제 적용 및 바이오필름 적절성 확인 12
Ⅲ. 연구 결과 15
1.DUWL에서 수집한 세균의 성장 곡선 15
2.DUWL 모델에 접종을 위한 세균 배양 15
3.바이오필름 분석 15
4.실험실 모델 확립 17
5.소독제 적용 및 바이오필름 적절성 확인 17
Ⅳ. 고찰 25
Ⅴ. 결론. 31
Ⅵ. 참고문헌 32
Ⅶ. 영문초록. 38
