미생물총기술(BFT)을 이용한 문치가자미(Limanda yokohamae)의 종묘생산과 양식생리학적 특성
Seedling Production of Marbled Sole, Limanda yokohamae using Biofloc Technology, and Bio-physiological Characteristics
- 주제(키워드) 문치가자미 , 미생물총 , BFT , 생리학적 , 조직학적 변화
- 발행기관 강릉원주대학교 일반대학원
- 지도교수 박기영
- 발행년도 2015
- 학위수여년월 2015. 2
- 학위명 박사
- 학과 및 전공 일반대학원 해양생물공학과
- 세부전공 수산양식
- 원문페이지 xii, 124
- 실제URI http://www.dcollection.net/handler/kangnung/000000007054
- 본문언어 한국어
초록/요약
In the study, seedling production of marbled sole Limanda yokohamae was carried out using Biofloc technology (BFT). The somatic growth of larvae to the juvenile stage was measured as well as the water quality of BFT rearing tanks for 129 days. Pathology tests were conducted on adults and dead juveniles that died during rearing; the presence of parasites was determined using microscopy, while tests for bacteria and viruses used selective media for Salmonella sp. and Vibrio sp. and RT-PCR, respectively. In addition, histological observations were conducted for pigment layers of the eye, epithelial cells of the intestine and the gastric gland of the stomach, the nuclei size of hematocytes, and oil droplets of the liver. Molasses, glucose, and sodium bicarbonate were used to manufacture Biofloc in 8-m-diameter tanks. These carbohydrate sources were added at 10 days after hatching (DAH) for the first, second, and third tanks. At 41 DAH, larvae began post-metamorphic settlement during the fourth and fifth tanks. Egg hatching rates ranged from 78.1 to 86.7% when attached to the bottom of tanks. The water depth of tanks just after attachment was maintained at 40–80 cm until hatching. The feeding schedule included enriched rotifers, Artemia, clam meat, copepods, and an artificial diet. Larvae and juveniles were also maintained as controls in flow aquaculture (FA) and were sampled for biochemical assays at 14, 21, and 44 DAH. Fertilized eggs hatched a large number of larvae at 170 and 205 h after fertilization. Growth rapidly increased during the first month after hatching to a body length of 10 mm. The average size of juveniles at 120 DAH was 45.7 mm. Body growth did not significantly differ between fish reared under FA and BFT during the period of 12 to 23 DAH. No pathogenic parasites, bacteria, or viruses were detected in adults or produced larvae and juveniles. The DNA content of FA larvae and juveniles increased to 0.55 ± 0.007 μg/larva at 21 DAH but did not increase thereafter. The DNA content of BFT fish increased to 1.08 ± 0.180 μg/larva at 53 DAH. The RNA/DNA ratio of BFT fish was stable after a rapid increase to 28 DAH. ALPase activities of BFT fish at 21 DAH were higher (up to 19.8 ± 1.00 U/larva) than those of FA fish. The α-amylase activity of FA fish was higher than that of BFT fish at 21 DAH, but activity levels at 14 and 44 DAH were similar for both fish types. Trypsin activity levels of FA fish were similar to those of BFT fish at 14 DAH, whereas levels at 21 DAH were higher (45.3 U/larva) for FA fish; however, trypsin activity of FA fish then decreased to 101.6 ± 6.63 U/larva, which was lower than that of BFT fish. Chymotrypsin activity levels of FA fish were similar to those of BFT fish at 14 and 53 DAH, but values for BFT fish were higher at 21 DAH. Lipase activity of FA fish was similar to that of BFT fish at 14 and 21 DAH, but activity of BFT fish was higher by 53 DAH. In addition, lysozyme activity was detected only in BFT fish at 14 and 21 DAH. Histological observations of marble sole larvae and juveniles reared using BFT indicated the presence of five complete retinal layers at 34 DAH. In addition, the thickness of the intestinal wall increased beginning at 6 DAH, and the density of intestinal epithelial cells had increased to 1750 cells/100 μm2 by 34 DAH. The thickness of the stomach wall continuously increased up to 127 DAH, where as the density of cells of the gastric gland in the stomach wall tended not to increase after 77 DAH. The nuclei size of hematocytes increased until 77 DAH, and the accumulation of oil droplets in liver tissue continuously increased until 127 DAH. Overall, the somatic growth of marbled sole L. yokohamae reared using Biofloc technology rapidly increased from 32 to 51 DAH, and the activities of digestive enzymes also increased during the same period. In addition, somatic growth accelerated during this post-hatching period, as the functions of the eye, intestine, and stomach had completely developed. By characterizing the somatic growth and digestive enzyme activities of larval and juvenile marbled sole, rearing by BFT have identified crucial benefits. In particular, the increased digestive enzyme and cellular activities resulted in enhanced somatic growth by 34 DAH in marbled sole reared using BFT compared to those reared using FA.
more목차
Ⅰ. 서 론
Ⅱ. BFT를 이용한 문치가자미 종묘생산
1. 서론
2. 재료 및 방법
3. 결과
4. 고찰
Ⅲ. BFT와 유수식에서의 문치가자미 자치어의 양식생리학적 비교
1. 서론
2. 재료 및 방법
3. 결과
4. 고찰
Ⅳ. BFT에서 사육된 문치가자미 자치어의 조직학적 변화
1. 서론
2. 재료 및 방법
3. 결과
4. 고찰
Ⅴ. 종합 고찰
Ⅵ. 국문 요약
Ⅶ. 참고문헌
