박하추출물의 Transforming Growth Factor-β Activated Kinase1 하향조절 활성
Transforming Growth Factor-β Activated Kinase1 down-regulating activity in the extract from Mentha arvensis var. piperascens
- 주제(키워드) 도움말 Transforming growth factor-β activated kinase1 (TAK1) , Mentha arvensis var. piperascens , Anti-inflammatory , Recycling HPLC.
- 발행기관 강릉원주대학교 일반대학원
- 지도교수 이창호
- 발행년도 2014
- 학위수여년월 2014. 8
- 학위명 석사
- 학과 및 전공 일반대학원 생물학과
- 원문페이지 iii, 27 p.
- 실제URI http://www.dcollection.net/handler/kangnung/000000006475
- 본문언어 영어
초록/요약
Plants have been used as foods and traditional medicines from a long time ago. It is generally regarded that plants have various positive effect for us such as anti-oxidant, anti-cancer, and anti-inflammation. For an example, peppermint (Mentha arvensis var. piperascens ) was used as a fever remedy, pain killer, and detoxificant. Since the beneficial effects are often related with anti-inflammatory activity, I examined the effects of peppermint extract on the immune responses using the cultured macrophages as a model system. Transforming growth factor-β activated kinase1 (TAK1) is an intracellular molecule that plays a key role in inflammation response by activating the down-stream NF-κB and AP-1 signaling pathway. There were reports that some natural ingredients affect the TAK1 signaling. The aim of the present study was to examine that if there is a TAK1 regulating activity in the peppermint extract using RAW 264.7 macrophage cells. The results showed that the extract from Mentha arvensis var. piperascens showed a strong down-regulating activity against TAK1 protein level. In addition, the peppermint extract down-regulated TAK1 level in a dose-dependent manner. The down-regulating activity was appeared from 3 h after the treatment, and continued until 48 h. Moreover, the TAK1 down-regulating activity was partially isolated using recycling HPLC. This study suggested that the anti-inflammatory effect of peppermint extract might be related with its TAK1 down-regulating activity.
more목차
Ⅰ. Introduction 1
Ⅱ. Materials and Methods 3
1. Plant materials 3
2. Extraction and partial purification isolation 3
3. Cell culture and treatment 4
4. Cytotoxicity test 4
5. Western blot analysis 5
6. Real-time quantitative PCR (QPCR) 6
Ⅲ. Results 7
1. Effect of the extract from Mentha arvensis var. piperascens on cell viability 7
2. Effect of the extract from Mentha arvensis var. piperascens on TAK1 protein level : time-course and dose-dependent study 9
3. The mechanism study for TAK1 down-regulation 12
4. Fractionation of TAK1 down-regulating activity using ultrafiltration and HPLC 15
Ⅳ. Discussion 19
Ⅴ. Abstract in Korean 21
Ⅵ. Reference 22
