해조 중의 향균성 물질의 분리 및 동정
Isolation and Structure Identification of Antibacterial Substances from Seaweeds
- 발행기관 강릉원주대학교 일반대학원
- 지도교수 趙舜榮
- 발행년도 2010
- 학위수여년월 2010. 2
- 학위명 석사
- 학과 및 전공 일반대학원 해양바이오산업협동과정
- 실제URI http://www.dcollection.net/handler/kangnung/000000006281
- 본문언어 한국어
초록/요약 도움말
This test has performed a search for anti-bacterial substance of marine algae and studied the essence of the substance. The organic layer of the natural seaweed from the East Sea had a slight anti-bacterial activities against bacteria such as E. coli and S. aureus. while the organic layer of porhyra denata from Wando had a slight anti-bacterial activities against bacteria such as S. aureus In addition, the water layer of the natural laminaria from Sageunjin had a slight growth inhibiting effect on fungi such as C. albicans and a strong growth inhibiting effect on bacteria such as E. coli and S. aureus. The anti-bacterial compounds are separated from the methanol extract of the indigenous laminaria from Sageunjin through the vacuum chromatography. Two compounds having the anti-bacterial activity were separated by Sep-PAK(C18) and C8 column oriented HPLC.The anti-bacterial compounds were supposed to be 5,7,8-trihydroxyflavone and 5,7,8-trihydroxyflavone 7-O-glucuronide by comparing the absorption UV-Visible spectrophometry to several flavonoids.
more목차 도움말
List of tables ⅴ
List of figures ⅵ
Abstract ⅶ
Ⅰ. 서 론 1
Ⅱ. 재료 및 방법 4
1. 실험재료 4
2. 실험방법 4
(1) 해조류 추출물의 분리정제 4
(2) 향균활성 측정 6
(3) VLC 분획구간별 향균활성 측정 6
(4) TLC(Thin layer chromatography)에 의한 분획 9
(5) 기기분석에 의한 분획된 물질의 동정 9
Ⅲ. 결과 및 고찰 10
1. 향균활성 검색 10
2. 기기분석을 통한 향균활성 물질의 분리 16
3. 향균활성 물질 본체 검색 21
Ⅳ. 결론 및 요약 25
Ⅴ. 참고 문헌 27
