면역결핍 마우스에서 인간 이하선 선양낭성암종 동위종양과 이소종양의 생물학적 특성에 관한 실험적 연구
AN EXPERIMENTAL STUDY ON THE BIOLOGICAL BEHAVIOR OF ORTHOTOPIC VERSUS ECTOPIC TUMORS OF HUMAN PAROTID ADENOID CYSTIC CARCINOMA IN ATHYMIC MICE
- 주제(키워드) Adenoid cystic carcinoma , Orthotopic tumor model , Lung metastasis , Cellular and molecular mechanisms
- 발행기관 江陵大學校 大學院
- 지도교수 朴瑛郁
- 발행년도 2008
- 학위수여년월 2008. 2
- 학위명 박사
- 학과 및 전공 齒醫學科
- 세부전공 口腔顎顔面外科學 專攻
- 원문페이지 48 p.
- 본문언어 한국어
초록/요약
Purpose: Adenoid cystic carcinoma (ACC) is a relatively rare tumor that arises in glandular tissues of the head and neck region and sometimes has a protracted clinical course with perineural invasion and delayed onset of distant lung metastasis. Treatment failure of salivary ACC is most often associated with perineural and hematogenous tumor spread. However, very little has been known about the cellular and molecular mechanisms of perineural invasion and hematogenous distant metastasis of parotid ACC. This study was designed to develop an orthotopic tumor model of parotid adenoid cystic carcinoma in athymic nude mice. Experimental Design: A melanoma cell line was injected into the parotid gland of athymic mice to determine whether such implantation was technically feasible. A parotid ACC cell line was then injected into the parotid gland or the subcutaneous tissue of athymic mice at various concentrations of tumor cells, and the mice were thereafter followed for development of tumor nodule. The tumors were examined histopathologically for perineural invasion or regional or distant lung metastasis. We used an oral squmous cell carcinoma cell line as control. Results: Implantation of tumor(melanoma) cell suspension into the parotid gland of nude mice was technically feasible and resulted in the formation of parotid tumors. The parotid ACC cell line, ACC3 showed no significantly higher tumorigenicity, but showed significantly higher lung metastatic potential in the parotid gland than in the subcutis. In contrast, a mucosal squmous cell carcinoma cell line didn’t show significantly higher lung metastatic potential in the parotid gland than in the subcutis. ACC tumors established in the parotid gland seemed to demonstrate perineural invasion of facial nerve, needs further study. Furthermore, immunohistochemical staining demonstrated higher expression of major angiogenic factors (VEGF, bFGF, and MMP-9) in the orthotopic parotid tumors than in ectopic subcutaneous tumors. Conclusion: An orthotopic tumor model of salivary ACC in athymic nude mice was successfully developed that closely recapitulates the clinical situations of human salivay ACC. This model should facilitate the understanding of the cellular and molecular mechanisms of tumorigenisis and metastasis of salivary ACC and aid in the development of targeted molecular therapies of salivary ACC.
more목차
I. 타액선 암종의 임상적 특성 = 1
II. 타액선 암종의 종양생물학적 특성 = 3
III. 면역결핍 마우스에서 종양모델의 확립 = 8
IV. 연구목표 = 10
V. 연구재료 및 연구방법 = 11
VI. 연구결과 = 18
VII. 총괄 및 고찰 = 24
VIII. 요약 및 결론 = 30
참고문헌 = 31
Tables = 39
Figure legends = 44
