해양 심층수를 활용한 유용 미세조류의 성장 및 지방산 조성
Growth and Fatty Acid Composition of Useful Microalgae cultivation using Deep Ocean Water
- 발행기관 강릉대학교 일반대학원
- 지도교수 박기영
- 발행년도 2007
- 학위수여년월 2007. 2
- 학위명 석사
- 학과 및 전공 해양생명공학과
- 원문페이지 vii, 36 p.
- 본문언어 한국어
초록/요약
A study was carried out to investigate the culture potentialties of microalgae using deep ocean water(DOW). Isochrysis galbana is haptophyceae which, for its good nutritive charateristics, is of substantial interest in aquaculture, principally to feed mollusk larvae, as well as fish and crustacean larvae. Also Spirulina platensis is Cyanophyceae which, includes valuable chemical such as carotenoid, antibiotics, fatty acids and antioxidant vitamins. Results of batch culture for seven days, growth of Isochrysis galbana was higher at DOWC(DOW added ferric EDTA) than at SSW(surface sea water) but specific growth rate showed no significant difference(P > 0.05) between culture fluids. Also fatty acid component(%/total fatty acid) is followed by saturated fatty acid(80.12~92.35%), monounsaturated fatty acid(4.20~16.98%), polyunsaturated fatty acid(2.69~4.55%). n3 and n6 unsaturated fatty acid components was not significantly differed between DOW, DOWC and SSWC(SSW added ferric EDTA). In Spirulina platensis cultivation, cell density was increased proportional to time in all experiments but the specific growth rate(SGR, %/day) was shown specific growth rate showed no significant difference(P > 0.05) between culture fluids. The growth of S. platensis was shown DOWdiC(DOWdi added ferric EDTA) higher than DOWdi(dilution of DOW) and SSWdiC(SSWdi added ferric EDTA). Also fatty acid component(%/total fatty acid) is followed by saturated fatty acid(78.01~84.38%), monounsaturated fatty acid(3.96~8.40%), polyunsaturated fatty acid(11.66~15.23%). Palmitic acid(C16:0) was shown the best contents to 62.76 ~ 67.72% in total fatty acid, and the specific growth rate(SGR, %/day) was shown specific growth rate showed no significant difference(P > 0.05) between culture fluids. Also eicosapentaenoic acid(EPA, C20:5n3) was detected trace contents in DOW and SSW Nutritive salts was shown higher DOW than SSW, Also DOW was shown mineral metals as Mg, Ca, K, Fe higher than medium. However in the character of DOW was shown growth higher or similar than SSW, but aspect of production of DHA and EPA is noneffective. Consequently, using EDTA to make up for the weak points. the growth of microalgae was shown higher sea water added EDTA than natural sea water. Therefore, I think, DOW could be possible economical culture fluid of microalgae but now, devised effective culture environment such as added to EDTA or nutritive salts.
more목차
Ⅰ. 서 론 = 1
Ⅱ. 재료 및 방법 = 3
1. 해양미세조류 = 3
2. 배양 및 회수 = 3
1) 배양 조건 = 3
2) 세포의 회수 = 4
3. 분석 방법 = 7
1) 일간비성장율 = 7
2) 배양액의 성분 분석 = 7
3) 지방산메틸에테르 조제 = 7
4) 지방산 분석 = 8
5) 통계 분석 = 8
Ⅲ. 결 과 = 11
1. 배양액의 성분 조성 = 11
1) 배양액의 영양염류 = 11
2) 배양액의 미량원소 = 14
2. 미세조류의 성장 = 17
1) Isochrysis galbana의 성장 = 17
2) Spirulina platensis의 성장 = 20
3. 미세조류의 지방산 조성 = 23
1) Isochrysis galbana의 지방산 조성 = 23
2) Spirulina platensis의 지방산 조성 = 25
Ⅳ. 고 찰 = 27
1. Isochrysis galbana의 배양 = -5
2. Spirulina platensis의 배양 = -5
3. 결론 = -5
Ⅴ. 요 약 = 31
Ⅵ. 참고문헌 = 33
감사의 글 = -5
