Effects of dietary carbohydrate sources and levels on growth and body composition of juvenile sea cucumber (Stichopus japonicus)
- 발행기관 Kangnung National University Graduate School
- 지도교수 Jeon, Joong-Kyun
- 발행년도 2007
- 학위수여년월 2007. 2
- 학위명 석사
- 학과 및 전공 Department of Marine Bioscience & Technology
- 원문페이지 30 p.
- 본문언어 영어
초록/요약
본 연구는 해삼 사료의 적정 탄수화물 종류 및 첨가량을 알아보기 위하여 수행되었다. 모든 실험사료에 소맥분을 10%씩 첨가한 후, 탄수화물원으로 소맥분, α-전분 및 알긴산를 각각 15% 및 30% 첨가하여 6종류의 실험사료를 제조하였다. 그리고 해조류(지충이) 실험구를 추가하여 실험사료들과 비교하였다. 평균 체중 770 mg의 해삼을 각 수조마다 70마리씩 3반복으로 수용하여 10주간 사육실험하였다. 사육 실험이 끝난 후, 해삼의 생존율은 60% 이상이었으며, 실험구간에 유의적인 차이는 없었다. 증중량은 사료내 탄수화물원(P<0.01) 및 함량에(P<0.001) 유의적인 영향을 받았으며, 사료내 탄수화물이 증가함에 따라 감소하는 경향이 나타났다. 15% 소맥분 실험구가 가장 높은 증중량을 나타냈지만, 15% 전분 및 지충이 실험구와는 유의적인 차이가 없었다(P>0.05). 사육실험 종료 후, 해삼의 수분(91.2－91.8%), 단백질(3.2－3.5%) 및 지질(0.13－0.29%) 함량은 탄수화물원 및 함량에 영향을 받지 않았지만(P>0.05), 회분함량은(3.4－3.8%) 탄수화물원에 따라 차이를 보였다(P<0.05). 이상의 결과로부터, 본 실험에 사용된 탄수화물원 중에서 해삼은 소맥분 및 알긴산을 α-전분보다 잘 이용하며, 사료내 적정 탄수화물 함량은 25%로 판단된다.
more초록/요약
A feeding trial of three dietary carbohydrate sources (wheat flour, α-starch and sodium alginate) and two dietary carbohydrate levels (15% and 30%) factorial design with three replications was conducted to investigate the proper dietary carbohydrate source and its level on growth of juvenile sea cucumber. In addition, sea cucumber fed the formulated diets was compared with the sea cucumber fed the dried Sargassum thunbergii. Juvenile sea cucumber (average body weight of 770 mg) were fed once a day with the experimental diets for 10 weeks. After feeding trial, survival rate was above 60 % but not significantly different among the groups. Mean weight gain was significantly affected by both dietary carbohydrate sources (P<0.01) and carbohydrate levels (P<0.001), and tended to decrease as dietary carbohydrate level increased. Mean weight gain of sea cucumber fed the diet containing 15% wheat flour was ighest, but not significantly different from those of sea cucumber fed the diet containing 15% sodium alginate and dried Sargassum thunbergii (P>0.05). No significant difference as observed in the contents of moisture (91.2－91.8%), crude protein (3.2－3.5%) and lipid (0.13－0.29%) in the whole body. Ash content (3.4－3.8%) was significantly affected by dietary carbohydrate source (P<0.05), and ash content of sea cucumber fed the diet containing 30% α-starch significantly (P<0.05) higher than that of sea cucumber fed with the diets containing 15% and 30% sodium alginate. Based on the results of this study, sea cucumber utilized wheat flour and sodium alginate more efficiently than α-starch and the diet containing 25% carbohydrate is recommended for growth juvenile sea cucumber.
more목차
1. Introduction = 6
2. Materials and methods = 8
2.1. Experimental diets = 8
2.2. Experimental animal and feeding trial = 11
2.3. Sample collection and chemical analysis = 11
2.4. Statistical analysis = 14
3. Results = 15
4. Discussion = 22
Abstract in Korean = 25
Acknowledgments = 26
References = 27
